荧光共振能量转移介导的上转换荧光探针用于EBV-DNA的检测研究

Epstein-barr virus (EBV) was found the earliest virus to be associated with human tumor, early and accurate detection of the DNA sequences of EBV is extremely important for the disease diagnosis and treatment. The current DNA detection methods in clinical application suffer from high cost and they are prone to false positive results. This project aims to design and construct novel up-conversion nanoprobes for highly sensitive EBV-DNA detection based on FRET (Fluorescence Resonance Energy Transfer). Up-conversion nanoparticles (UCNPs) and organic dyes /Au nanoparticle will act as the energy donor and the energy acceptor in the FRET system, respectively. This study will not only extend the applications of rare earth up-conversion nanophosphors in the biological applications, but also enrich EBV-DNA detection. Meanwhile, it has the reference value for DNA related disease diagnosis and treatment.

EB病毒(Epstein-Barr virus, EBV)是最早被发现与人类肿瘤相关的病毒，及早和准确的检测引起该疾病的DNA序列对于EBV患者的诊断和治疗具有重要意义。针对目前临床中EBV-DNA检测技术要求高、误差大、易出现假阳性结果等问题，本项目提出以荧光共振能量转移原理(fluorescence resonance energy transfer, FRET)为介导，设计构筑基于以稀土上转换发光纳米粒子为能量给体，有机染料/金纳米为能量受体的DNA 纳米荧光探针，筛选高灵敏度、专一识别EBV-DNA的荧光探针，实现EBV-DNA的痕量检测。本研究将拓展上转换发光材料在生物方面的应用，丰富EBV-DNA的检测手段，对与DNA相关疾病诊断和治疗具有参考价值。

项目按照研究计划，利用荧光共振能量转移介导的上转换荧光探针实现EBV-DNA的检测研究。发表科研论文5篇，其中SCI收录4篇，中文核心期刊1篇，授权国家发明专利1项。成果1：基于上转化材料修饰的探针，利用荧光共振能量转移机理，实现EBV-DNA的检测。成果2：合成苯甲酰肼衍生物，实现Mg2+的检测。成果3：合成苯甲酰肼衍生物，在不同pH条件下，分别实现Mg2+和Al3+的检测，该研究为首次使用一种探针同时实现Mg2+和Al3+的检测。成果4：合成罗丹明B类荧光探针，实现细胞内Hg2+的定性检测。成果5：合成罗丹明B类荧光探针，实现生物体内Al3+的定量以及定性检测，该研究对推动生物体内的定量检测具有推动作用。成果6：合成罗丹明B类荧光探针，实现Fe3+的检测。成果7：对天然产物壳聚糖进行修饰，得到一种Al3+选择性荧光探针，已经授权国家发明专利。