荧光共聚焦技术在生物体内Al3+离子检测中的应用

Aluminum is a nonessential element in human body. Because of the biological enrichment effect of this element, great harm was brought to human body and other organisms. Therefore, many researchers focused on the detection/monitoring of Al3+. Among the methods for the detection of Al3+, fluorescence probe technique, using optical signal as output signal, has small damage or without damage for the detection of target substances in the body. However, due to the Al3+ coordination properties, the design and application of Al3+ fluorescent probe is always a difficult problem in academic circles. Based on our previous research, this project focuses on a breakthrough in the following three aspects: 1) the independent design of Al3+-selective fluorescence probe; 2) realize the quantitative detection of Al3+ in the living cells; 3) track the probe in the cells and locate the Al3+ in cells by counterstaining technology.

铝是人体非必需元素，但是由于该元素具有生物富集效应，给人体以及其它生物带来极大的危害。研究表明，老年痴呆症与Al3+在人体神经系统的富集有很大关系。因此，Al3+的检测/监测一直是研究的热点。在众多Al3+的检测方法中，荧光探针技术由于采用光信号作为输出信号，可实现损伤小、甚至无损伤地达到对生物体内目标物质的荧光成像检测，达到目标物质在生物体内的定性检测。但是，由于受到 Al3+配位性能的限制，Al3+荧光探针的设计及应用一直是学术界的难点。本项目在前期研究基础上，主要工作体现在以下三个方面：1）自主设计高选择性Al3+荧光探针；2）突破目前仅限于细胞内Al3+定性检测的现状，实现细胞体内Al3+的定量检测；3）利用复染技术，跟踪探针进入细胞体内的程度，实现细胞体内Al3+的定位检测。

本项目对设计合成Al3+离子荧光探针，实现该物质在生物体内外的定性及定量检测。具有选择性的荧光探针进行设计合成与筛选截至结题日期，合成目标离子荧光探针四个，测试结果令人满意。同时合成Cu2+、Zn2+荧光探针3个。课题组成员也对重金属形态分析进行了研究。发表科研论文11篇，其中SCI收录3篇；申请国家发明专利4项，已获得国家授权发明专利1项；出版专著和教学参考书3部。该项目的研究，为设计合成新型的荧光探针以及探针在重金属离子检测方面提供了一定的科学依据。