华北典型地区育龄妇女多环芳烃的体外及体内暴露水平研究

Polycyclic aromatic hydrocarbons (PAHs) are a group of important environmental organic pollutants in north China, which have potential adverse effects on the health of childbearing-age women and their fetal development. To date, the comprehensive assessment of their internal and external exposure levels to PAHs are critically lack. We planned to recruit 80 childbearing-age women in the area of Baoding City of north China. In the heating and non-heating seasons, we will conduct a three-month follow-up study, respectively. The typical environmental samples of diet and inhalation exposure pathways were collected, which were selected based our previous study, as well as the morning urine and hair samples of cildbearing-age women. The patterns of their diet habit and daily activity behaviors were separately investigated by questionnaires and cell phone APP software. The PAH concentrations in atmospheric and dietary samples were analyzed to assess their external PAH exposure levels, and PAHs or OH-PAHs in hair and urine samples to indicate their internal PAH exposure levels. The reliable indicators for the overall PAH intake can be screened by investigating the relation between external and internal exposure levels. We also planned to confirm the reliability of the observed relations from human study by a rat model. Our proposal is featured by the interdisciplinary study of environmental science, animal model, and epidemiology. We aimed to assess the PAH exposure levels of childbearing-age women in north China with high accuracy and screen the reliable indicators for the overall PAH exposure level. Our study results can provide strong scientific and technical supports for making public health policies to protect the health of childbearing-age women in north China.

多环芳烃（PAHs）是我国华北地区环境中一类重要的有机污染物，对育龄妇女健康及其胎儿发育具有潜在危害。目前对其PAHs体外及体内暴露特征的联合研究较为缺乏。本项目拟在华北保定地区募集100名育龄妇女，在采暖期和非采暖期分别进行连续3个月的随访调查。采集膳食和呼吸中具有代表性的环境样品，以及妇女晨尿和头发标本。使用问卷和手机软件分别调查妇女膳食结构和日常行为模式。分析环境样品中PAHs浓度，以评估妇女PAHs的体外暴露水平。分析头发和晨尿中PAHs或OH-PAHs浓度，以指示PAHs的内暴露水平，并分析体内外暴露的相关性。开展大鼠实验进一步确认人群研究中的观察到的相关关系的可靠性。本研究融合环境科学、动物模型和流行病学研究手段，以期准确评估华北地区育龄妇女的PAHs暴露水平，筛选出能代表PAHs综合暴露水平的可靠指标。研究结果可为制定保护育龄妇女身体健康的相关公共卫生政策提供科学技术支撑。

多环芳烃（PAHs）具有潜在的生殖毒性，孕早期是胎儿较为敏感的环境污染暴露窗口，因此，准确评价我国育龄妇女的PAHs暴露特征非常重要。本课题主要针对华北地区育龄妇女人群，探讨其对PAHs的内、外暴露特征及主要影响因素，并筛选能指示PAHs暴露水平的可靠生物标志物。主要研究如下：（1）建立生物标本中PAHs分析方法。申请人克服生物样品基质对定量分析的干扰，开发了针对不同标本的同步分析方法，比如血液、尿液和头发等，有效支撑了人体生物标本检测分析的需求。（2）评价人群的PAHs内暴露水平。本研究中共检测了晨尿样本中13种单羟基PAHs产物。发现室内空气净化器干预可以显著降低室内PM2.5及其PAHs组分的浓度，尿液中OH-PAHs含量可以作为表征短期PM2.5及其PAHs相关组分暴露水平的内暴露标志物。（3）评价人群的PAHs外暴露水平。本研究中共检测PM2.5中41种PAHs，包括24种母体、12种硝基取代和5种含氧的PAHs。研究期间所采集的室内PM2.5样本中PAHs及其衍生物检出率均高于81%，发现6环以内PAHs是室内PM2.5中PAHs的主要组分。同时，分析了各类食物中 PAHs浓度，发现空气 PAHs 污染严重的满城地区，城乡食物的PAHs 污染也较为严重。（4）筛选人体中PAHs及其他典型环境暴露物质的暴露标志物。PM2.5及PAHs的外暴露与尿液OH-PAHs间的关联分析结果发现，室内PM2.5中部分PAHs及其衍生物也与尿液中9-OH-PHE间存在显著正关联。动物暴露模型表明，尿液和头发标本，相对于血液，更适用于指示人体对PAHs的外暴露水平。此外，基于项目内容和产出，积极开展合作研究，包括筛查影响不良妊娠结局、生殖发育、脂质代谢、血压和流感等重要健康问题的环境因素，以及参与新冠疫情防控。该项目发表中英文论文共30篇，其中SCI JCR Q1区21篇。