基于扫频OCT相位信息的微循环成像方法与应用研究

The abnormality of microcirculation is highly related to lots of diseases, and usually regarded as the source of all diseases. The imaging study of microcirculation not only improves the understanding of the pathology of the related diseases, but also contributes to the diagnosis and the treatment evaluation. However, the currently available imaging method is greatly limited. Thus, a label-free, high resolution, three dimensional, optical imaging techniques of microcirculation is of paramount importance for scientific study and clinical application. This project aims to the optical imaging of microcirculations based on the phase information of swept source OCT and its applications. A method for precise calibration and dynamic compensation of swept source spectrum will be developed. And a method for compensating bulk tissue motion and extracting local flow signal will be realized. An optical imaging system of microcirculation based on the phase information of swept source will be built, providing the three dimensional rendering of blood perfusion and the quantitative evaluation of blood flow. The preliminary applications will be studied in several diseases, such as retinal disease and tumor. This study will not only improve the development the OCT functional imaging techniques, but also provide a novel approach for the diagnosis and treatment of diseases with vascular involvement.

微循环障碍与诸多疾病的发生发展存在紧密的关联性，甚至有“百病之源”一说。微循环的成像研究不但可以深入了解相关疾病的病理机制，而且可以用于疾病的诊断（特别是早期诊断）与治疗效果的评价。但是，目前可以用于微循环活体成像的方法却十分受限。因此，发展无标记、高分辨、三维光学微循环成像技术具有极其重要的科学研究与临床应用价值。本项目将基于扫频OCT相位信息开展微循环成像的方法与应用研究，发展扫频光谱精确标定与动态补偿的方法，发展整体组织抖动补偿与局部血流信号提取的方法，建立基于扫频OCT相位方法的微循环成像系统，获得组织毛细血管分布的三维形态结构以及血流量等生理功能信息，开展诸如眼底视网膜疾病、早期癌症等疾病的应用研究。本项目的成功实施不仅有助于推动OCT功能成像技术的发展，而且为诸多疾病的诊断和治疗提供新的技术手段。

本项目旨在发展基于扫频OCT相位的微循环成像方法及系统的研究，建立扫频OCT系统，研究大量程活组织抖动的相位补偿技术，开展微循环成像应用研究，实现血流参数的定量测量。项目完成了扫频光谱精确标定与动态补偿，研究了整体组织抖动补偿与局部血流信号提取的方法，搭建了扫频OCT系统。研究了无标记血流造影机制，提出了基于复数互相关的微小血流运动监测方法，研究了独立散射样本的并行采集，提出了多角度并行采集方法和基于混合多样性策略的大样本采集方法，实现了高灵敏度的微循环成像。研究了血流对比度增强方法，提出了基于深度自适应阈值和基于Hessian矩阵图形滤波的血流增强方法。基于建立的扫频OCT系统，开展了微循环血流成像在鼠中风模型中的应用试验，获得了高质量的三维血管分布图像，实现三维血管网的动态表征与定量分析。本项目发展的无标记高分辨三维成像技术，具有重要科学研究与临床应用价值。