贵金属纳米团簇的荧光增强及其在卵巢癌早期诊断上的应用

Ovarian cancer was mostly found at advanced stage. The delay is mainly due to the drawbacks of the diagnosis strategies, which is difficult to be accepted by common people. For the early diagnosis of ovarian cancer, test strips will show great potentials in the future. However, the current paper based strips such as gold labeled method, quantum dot immunochromatographic strip still describe disadvantages including inefficient detection limit, toxicity and high cost, etc. Thus, for real applications, the further development of the techniques is in requirement. Nanoclusters are with tiny sizes, low toxicity and cost effective properties. They have great potentials to be applied in the field of medical examination. However, due to the unresolved drawbacks, few works have been contributed to the fabrication of strips with nanoclusters for the detection. In this project, various strategies are investigated for improvement of the brightness and stability of the fluorescence for the nanoclusters. Thus a new type of materials with great advantages will be contributed to the early diagnosis of ovarian cancer. Versatile sensing strategies for detection of tumor markers will be investigated. Then, the paper based test strip will be developed based on the modified nanoclusters and the as designed probes. The sensing strategies and the corresponding products for our work will describe great importance in the area of early ovarian cancer diagnosis.

卵巢癌常在中晚期才被发现，主要原因是目前的诊断技术尚不被轻易采纳。在卵巢癌早期诊断领域，试纸检测是一种较易被普遍接受的手段。而现有的试纸，包括金标法试纸、量子点标记免疫层析试纸等尚有不足之处，如检出限有待提高，毒性大，成本相对较高，选择性不强等。这项技术仍有待于发展。纳米团簇是一种尺寸极小的纳米材料，毒性小，制备成本低廉，在医学检测领域具有较普通纳米材料为基体材料的诊断器件优越的潜力。但是，由于其不足之处尚未被完全改善，这种材料几乎未在医学诊断器件中得到应用。我们通过改善纳米团簇的荧光产率与稳定性，为早期卵巢癌诊断技术领域引入有效的新型材料。利用纳米团簇可以发射不同波长的荧光的性质，设计可满足多种肿瘤标志物的高选择性识别的通用探针策略。以探针原理为基础，制备相应的纸基检测器件。所设计的探针策略及试纸产品将对卵巢癌早期诊断作出重大贡献。

卵巢癌常在中晚期才被发现，主要原因为目前的诊断技术仍有缺陷，尚不被轻易采纳。在卵巢癌早起诊断领域，研究较易被大众接受的方便手段，将对卵巢癌的筛查产生重大意义。现有的早期筛查方案，多为包括金标试纸、量子点标记免疫层析技术等。以上检测方案尚有不足之处，如检出限有待提高，毒性大，成本相对较高，选择性不强等。这些早期诊断技术仍有待于发展。纳米团簇是一种尺寸极小的纳米材料，毒性小，制备成本低廉，在医学检测领域具有较普通纳米材料为基体材料的诊断器件优越的潜力。但是，由于不满足荧光产率高或容易修饰的要求，这种材料几乎未在医学诊断器件中得到应用。我们通过改善纳米团簇的荧光产率，将其有效地应用于早期卵巢癌筛查领域。利用纳米团簇可以发射不同波长荧光的性质，设计区分卵巢癌样本的多种策略。在探针基础上，设计检测手段，将对癌症早期诊断作出重大贡献。