基于一体化侧流适体传感器的环境雌激素快速检测新方法

Environmental estrogens (EEs) pose a serious threat to the ecological environment and human health. It is of great significance to develop immediate and in situ rapid detecting methods for the control and reduction of EEs. This project focusses on the new mode and mechanism of separation and analysis in lateral flow sensing technology based on nucleic acid aptamer instead of antibody, and its application in the rapid screening and detection of EEs such as estradiol, ethylestradiol, diethylstilbestrol, isoflavone, coumestrol, pentachlorophenol, bisphenol and phthalate esters. The aim of this project is to design and develop some novel integrated or all-in-one lateral flow aptasensors (LFAs) with sample concentration, purification, separation and analysis functions, to evaluate the effect on detection by the following factors including detecting mode (such as sandwiching and competitive mode), signal reporting type (such as visualization, fluorescence or afterglow, chemiluminescence or bioluminescence) and the character of nitrocellulose membranes(such as brand and pore size), and to discuss the ideal working mode and separation mechanism of LFAs to get better selectivity, lower detection limit and wider quantitative range.

环境雌激素对生态环境和人类健康构成严重威胁，开发即时、原位快速检测方法，对于控制和削减环境雌激素具有重要意义。本项目旨在深入系统研究纯核酸型侧流适体传感技术中分离分析的新模式与新机理，及其在环境雌激素快速筛选与检测中的应用。拟以雌二醇、乙炔雌二醇、己烯雌酚、异黄酮、拟雌内酯、五氯化酚、双酚、酞酸酯类化合物等几类环境雌激素为分析对象，设计并构建集样品富集、纯化、分离、分析功能的新型一体化侧流适体传感器，探讨夹心型与竞争型侧流适体传感器的检测性能，考察显色、荧光或余辉、化学或生物发光等不同类型纳米粒子作为信号报告元件对选择性、检测限与定量范围的影响，研究不同品牌与孔径的硝酸纤维素膜对识别和分离性能的影响，探讨纯核酸型侧流适体传感器的理想工作模式与分离机理。本项目的实施能够为环境雌激素的即时、原位快速检测提供新方法。

本项目以雌二醇、双酚等环境雌激素为分析对象，发展了基于长余辉纳米材料、金属有机骨架、黑磷-金纳米复合物等多种材料的样品富集、纯化、分离、分析新方法，拓展了上述新材料的应用领域，尤其是在新型侧流传感领域中的应用，解决了环境雌激素快速分离、分析的若干关键问题，构建了多种新颖分离、分析装置。在Journal of Water Process Engineering，Talanta，Food Chemistry和ACS Applied Bio Materials等期刊上发表SCI论文11篇，发表北大中文核心期刊4篇，授权专利4项，获2021年中国商业联合会科技进步奖一项（2/10），完成了预定的研究目标。