脂联素信号通路在高脂诱导胰岛素抵抗中的作用及针刺干预机制研究

On the basis of previous researchs,this study forcuses on the other important machenism of insulin resestance - disturbance of lipid metabolism. In this study, we use the model rats with insulin resistance induced by high-fat diet as objects and pioglitazone as control, systematically observe acupuncture's regulating effect of lipid metabolism disorder in insulin resistance at multi-level, propose the hypothesis that improving lipid metabolism disorder is one of the most important ways for acupuncture reversing insulin resistance, the key molecular mechanism of this way is the AMPK-ACC/MAPK-PPARγ signal transduction pathway induced by adiponectin. This hypothesis would be verified by rigorous experimental design and advanced experimental technology. The conclusions of this study would supply scientific evidence for acupuncture reversing insulin resistance and regulating lipid metabolism disorder,and supply steady theory for acupuncture's treatment on the diseases related to insulin resistance, such as obesity, disbetes, cardiovascular and cerebrovascular cerebral diseases in clinic.

本项目在前期研究的基础之上，着眼于胰岛素抵抗发生、发展过程中的另一重要方面-脂质代谢紊乱。以高脂饮食诱导胰岛素抵抗模型大鼠为研究对象，以吡格列酮为对照，多水平、多层次地系统观察针刺对胰岛素抵抗脂质代谢紊乱的调节效应，提出 "针刺改善脂质代谢紊乱是针刺抗胰岛素抵抗的重要作用途径之一，而脂联素所激发的AMPK -ACC/MAPK-PPARγ信号转导通路是其发挥作用的关键分子机制"的科学假说。通过严谨的实验设计和先进的实验技术，观察针刺对胰岛素抵抗脂质代谢紊乱的改善作用，并探讨其分子信号机制；研究成果将为针刺改善胰岛素抵抗、调节脂肪代谢提供强有力的科学证据，并为临床应用针灸治疗高脂血症、肥胖、糖尿病及心脑血管等胰岛素抵抗相关疾病奠定坚实的理论基础。

本项目在前期研究的基础之上，着眼于胰岛素抵抗发生、发展过程中的另一重要方面—脂质代谢紊乱。以高脂饮食诱导胰岛素抵抗模型大鼠为研究对象，以吡格列酮为对照，多水平、多层次地系统观察针刺对胰岛素抵抗脂质代谢紊乱的调节效应，提出 “针刺改善脂质代谢紊乱是针刺抗胰岛素抵抗的重要作用途径之一，而脂联素所激发的AMPK -ACC/MAPK-PPARγ信号转导通路是其发挥作用的关键分子机制”的科学假说。通过严谨的实验设计和先进的实验技术，观察针刺对胰岛素抵抗脂质代谢紊乱的改善作用，并探讨其分子信号机制。结果显示，高脂饮食可有效诱导胰岛素抵抗模型，应用高胰岛素正葡萄糖钳夹技术可更客观反映胰岛素抵抗程度，针刺可通过改变脂联素所介导的AMPK-ACC/MAPK-PPARγ信号通路改善胰岛素抵抗。研究成果将为针刺改善胰岛素抵抗、调节脂肪代谢提供强有力的科学证据，并为临床应用针灸治疗高脂血症、肥胖、糖尿病及心脑血管等胰岛素抵抗相关疾病奠定坚实的理论基础。