IgY分子缓释型镧掺杂磷酸钙的制备及其防龋机制的基础研究

Due to the potential toxicity and side effects of fluoride, the exploration of fluoride-free anti-caries material with safety and effective property has become a significantly important field in the study of caries prevention. Ideal anti-caries material should possess such functions as anti-bacterial, re-mineralization and biocompatibility. However, there is no such efficient material which can achieve all the requirements at present. In this project, a novel multifunctional IgY protein molecule released lanthanum doped calcium phosphate has been designed for preparation of fluoride-free anti-caries material. In the experiment, calcium phosphate which is prepared under regulating of amphiphilic polymer PLA-mPEG is chosen as main constituents. The technique of lanthanum doping as well as IgY loading and releasing will be explored. Systematic experiments will be applied to explore the anti-caries properties and re-mineralization mechanism for dental caries. After the detailed characterization of the multiple-functional material on physical and chemical properties, the IgY protein molecule loading and releasing properties will be investigated and optimized. Then the preventive mechanism of the prepared material for dental caries will be studied with molecule biological methods such as RT PCR and Real-Time PCR, in the molecule level. Furthermore, the possible re-mineralization mechanism of the prepared material will be analyzed and clarified through the perspective of the structure, chemical phase and the change of chemical elements using the technique of TEM and ICP. Finally, animal experiments will be used to verify the anti-caries properties. All of the above studies will provide a theoretical basis for the preparation of calcium phosphate based multifunctional material and its application in the field of caries prevention.

防龋用氟化物具有潜在的毒副作用，构建安全有效的新型无氟防龋材料是龋病预防的重要方向。理想的防龋材料应具备抑菌、提高釉质抗酸力、再矿化及良好生物相容性等功能，目前国内外报道的防龋材料尚不能满足上述要求。本项目拟在前期研究基础上，选择具有良好生物相容性和再矿化作用的纳米磷酸钙为主体材料，利用双亲性嵌段聚合物PLA-mPEG调控其结构和物相，探索通过稀土元素镧掺杂及抗龋特异性IgY分子的装载/缓释实现功能化的技术，以达到构建多功能新型无氟防龋材料的目的。本研究将通过系统实验设计，实现材料的可控制备和理化性能表征；探索和优化IgY分子的装载和缓释性能；采用Real-Time PCR等方法从分子水平探讨材料的抗菌机制；利用TEM、ICP等方法从结构、物相和元素变化等角度阐明材料的再矿化机制；开展动物实验验证材料的防龋效果，为构建基于纳米磷酸钙的新型多功能复合材料体系及其在防龋领域的应用提供理论基础。

防龋用氟化物具有潜在的毒副作用，构建安全有效的新型无氟防龋材料是龋病预防的重要方向。理想的防龋材料应具备抑菌、提高釉质抗酸力、再矿化及良好生物相容性等功能，目前国内外报道的防龋材料尚不能满足上述要求。本项目选择具有良好生物相容性和再矿化作用的纳米磷酸钙为主体材料，利用双亲性嵌段聚合物PLA-mPEG 调控其结构和物相，探索通过稀土元素镧掺杂及抗龋特异性IgY 分子的装载/缓释实现功能化的技术，构建了多功能新型无氟防龋材料。本研究通过系统实验设计，实现材料的可控制备和理化性能表征；探索和优化IgY 分子的装载和缓释性能；另外，在探索制备磷酸钙材料的过程中，我们证实了PLA -mPEG、生物大分子DNA以及反应体系的条件对磷酸钙结构和形态的调控作用，这也为优化制备技术提供了理论依据。