胎盘环状RNA circPLCG2-miR519a-PTEN作用轴调控胎儿出生体重的分子机制研究

Abnormal birth weight has great negative effect on fetus and pregnant women.The placenta trophoblast growth condition is one of the most important reasons influence birth weight, whose molecular mechanism regulating the placenta nourishes of cell growth has not been elucidated. The newly discovered circular RNA has participated in plenty of physiological adjusting processes. We tested the placenta of different fetal birth weight by utilizing gene chip technology on the early stage. The result showed that circular RNA circPLCG2 was highly related to different birth weight. Furthermore, bio-informatic analyses revealed that the circRNA PLCG2 may target placenta specific miR-519a to affect placenta trophoblast growth by endogenous competitive RNA.We hypothesize that circPLCG2 - miR519a - PTEN polarization is one of the main reasons to affect birth weight, whereas the mechanism still need further research.Our following research will illuminate the molecular mechanisms of circPLCG2 - miR519a - PTEN function axis on fetal birth weight through the luciferase report experiment and RNA precipitation technology. We hope our study has positive impact on preventing abnormal fetal birth weight and complications of pregnancy.

出生体重异常严重影响胎儿及孕妇的身体健康，胎盘滋养细胞生长状态是影响出生体重的重要原因，而调控胎盘滋养细胞生长的分子机制迄今仍未阐明。新近发现的环状RNA参与多种生理过程的调节，课题组前期利用基因芯片等高通量技术对不同出生体重胎儿的胎盘进行检测，发现环状RNA circPLCG2与胎儿出生体重的变化密切相关；通过生物信息学分析发现，circPLCG2可能通过吸附胎盘滋养细胞特异性微小RNA miR-519a，以内源竞争性RNA的方式调控PTEN的表达进而影响胎盘滋养细胞生长。我们推测circPLCG2-miR-519a-PTEN作用轴是影响胎儿出生体重的重要原因，而其作用机制尚需进一步研究。为此，本项目将通过荧光素酶报告实验、RNA免疫沉淀等技术，探寻并阐明circPLCG2-miR-519a-PTEN作用轴影响胎儿出生体重的分子机制，为预防及治疗胎儿出生体重异常和妊娠相关并发症提供新思路

出生体重异常严重影响胎儿及孕妇的身体健康，胎盘滋养细胞生长状态是影响出生体重的重要原因，而调控胎盘滋养细胞生长的分子机制迄今仍未阐明。新近发现的环状RNA 参与多种生理过程的调节，课题组前期利用基因芯片等高通量技术对不同出生体重胎儿的胎盘进行检测，发现环状RNA circPLCG2与胎儿出生体重的变化密切相关；通过生物信息学分析发现，circPLCG2可能通过吸附胎盘滋养细胞特异性微小RNA miR-519a，以内源竞争性RNA的方式调控PTEN的表达进而影响胎盘滋养细胞生长。我们推测circPLCG2-miR-519a-PTEN作用轴是影响胎儿出生体重的重要原因，而其作用机制尚需进一步研究。为此，本项目将通过荧光素酶报告实验、RNA免疫沉淀等技术，探寻并阐明circPLCG2-miR-519a-PT .EN作用轴影响胎儿出生体重的分子机制，为预防及治疗胎儿出生体重异常和妊娠相关并发症提供新思路