新的细胞周期蛋白p42.3在青海世居藏族胃癌发生发展中的作用及机制

Gastric cancer is one of the main causes of death in cancer in present, so look for the development of molecular markers in gastric cancer is a key method to solve the diagnosis, treatment and prevention of gastric cancer. Qinghai Province is one of China's high incidence of gastric cancer. Especially, the incidence and mortality of Tibetan ranks first in the country. So, to study the pathogenesis of gastric cancer in native Tibetan has an important significance. In our previous research, we found that p42.3 gene was involved in cell cycle regulation and found that its expression was specific in gastric cancer tissues but not normal gastric mucosa. Furthermore, overexpressing p42.3 in NIH3T3 and HepG2 cells have promoted significantly cell growth and tumorigenicity. All these data indicate that p42.3 plays an important role in tumorigenesis, suggesting that it may be a potential tumor biomarker. However, as a novel cell cycle protein, the mechanism of p42.3 involved in gastric cancer development is still unclear. Therefore, we intend to take advantage of confocal and two-dimensional electrophoresis of a variety of experimental methods to explore interacting protein and the signal transduction pathways of p42.3, study the mechanisms of p42.3 protein in the development of gastric cancer, for the application of p42.3 as a molecular marker in gastric provide theoretical and experimental evidence.

胃癌是世界上癌症的主要死因之一，因此寻找胃癌发生发展的分子标志物是胃癌诊疗及预防中亟待解决的卫生问题。青海省是我国胃癌高发省份，尤其藏族发病率致死率居全国之首，因此，以青海世居藏族人群来阐明胃癌发病机制具有重要意义。 我们前期实验发现p42.3蛋白参与了细胞周期调控并在胃癌组织中特异性表达，过表达p42.3于NIH3T3及HepG2后导致细胞活力和体外集落形成能力明显增强，并显著增强了裸鼠的致瘤发生率，这些结果表明p42.3基因在细胞增殖和肿瘤形成中具有重要作用，提示其可能是肿瘤发生发展中的重要分子标志物。但是，p42.3作为一种新的细胞周期蛋白，其参与胃癌发生发展的作用机制并不清楚。因此我们拟利用激光共聚焦及双向电泳等多种实验方法研究p42.3蛋白的共作用蛋白及可能参与的信号传导通路，明确p42.3蛋白在胃癌发生发展中的作用机制，为进一步应用p42.3蛋白作为分子标志物提供理论及实验依据。