基于二代测序的GGRS技术研究云南盐津乌骨鸡繁殖性状遗传机制

Yanjin black-bone chicken is one of the famous indigenous chicken breeds in Yunnan province. However, their reproductive performance is generally low. It may be an effective way to improve reproductive performances by genetic improvement combining molecular breeding and conventional breeding based on revealing the molecular genetic mechanism of reproductive traits of Yanjin black-bone chicken. At present, high-throughput sequencing technologh has become one of the most important methods to study molecular genetic mechanism for complex traits. So, this study wil use GGRS technology established based next-generation sequencing by applicant to study molecular and genetic mechanisms underlying reproductive traits of yanjin black-bone chicken, and perform the following tasks: (1) performing analysis of comparative genomics and positive selection signal; (2) performing genome-wide association analysis on chicken reproductive traits. The study focuses on SNPs, genes and gene sets (biological metabolic pathways, signal transduction pathways, regulatory networks, etc.) associated with reproductive traits. The study will contribute to more systematic and in-depth understand reproductive characteristic of yanjin black-bone chicken, and lay a theoretical foundation of molecular breeding for improving reproductive performance.

盐津乌骨鸡是云南著名的地方优良品种，但繁殖性能普遍较低。阐明盐津乌骨鸡繁殖性状的分子遗传机制，进而通过分子和常规育种技术相结合对盐津乌骨鸡进行遗传改良，可能是提升盐津乌骨鸡繁殖性能的一个有效途径。目前，高通量测序技术已成为复杂性状分子遗传机制解析的重要方法之一。有鉴于此，本项目拟利用项目申请人建立的基于二代测序的GGRS技术对盐津乌骨鸡的繁殖特性进行研究，拟开展以下工作：一是进行比较基因组学和正选择信号分析；二是进行鸡全基因组关联分析。这些工作旨在分析鉴定影响盐津乌骨鸡繁殖性状相关联的SNP、基因及基因集（生物代谢通路、信号传导通路、调控网络等），以期初步阐释盐津乌骨鸡繁殖性状的分子遗传机制。本项目的实施将有助于更系统、深入了解盐津乌骨鸡的繁殖特性，为开展盐津乌骨鸡的分子育种工作进而提升其繁殖性能奠定理论基础。

盐津乌骨鸡是云南著名的地方优良品种，阐明盐津乌骨鸡繁殖性状的分子遗传机制，进而通过分子和常规育种技术相结合对盐津乌骨鸡进行遗传改良，可能是提升盐津乌骨鸡繁殖性能的一个有效途径。基于此，本项目开展了下列研究工作：1. 针对鸡的基因组结构和特征，建立了基于二代测序技术的鸡简化基因组遗传标记检测方法；2. 利用所建遗传标记检测方法对参试乌骨鸡群体进行了遗传标记检测；3. 利用检测到的遗传标记联合繁殖性状表型数据进行了全基因组关联分析，识别了与乌骨鸡繁殖性状相关的候选SNP位点和候选基因。