建立基于前列腺特异性抗原检测的特异选择性生物传感体系在前列腺癌早期诊断中的基础研究

Prostate Specific Antigen (PSA) is an internationally recognized tumor marker widely used in early diagnosis, prognosis, efficacy evaluation and recurrence monitoring of prostate cancer. Meeting the practical problems of tumor markers detection, this project propose to establish a specific selective immuno biosensing system which is highly-sensitive, long-stable and low-detection-limited, with the specific antibody (Anti-PSA) as the molecular recognition element for the detection of PSA, as to the current problems in the detection of tumor markers. It is supposed to be a new modern biological method for the detection of PSA. Nonspecific adsorption of surface protein in conventional blood detection is a ubiquitous problem. This project propose to synthesis a kind of oligosaccharide derivative blocking agents to inhibit this phenomenon. Characteristics of the blocking agents shall be evaluated via the biosensing system in practical experiments, based on traditional analysis methods. The blocking agents help optimize the biosensing system for the detection of PSA tumor marker and finally establish a biosensing system of excellent specificity, high sensitivity, good stability, wide detection range and low detection limit.The study shall promote the application of biosensing technique in laboratory medicine.

前列腺特异性抗原（PSA）是国际公认的在前列腺癌的早期诊断、预后判断、疗效评价和复发监测中广泛使用的肿瘤标志物。本项目针对目前肿瘤标志物检测中存在的实际问题，利用现代生物传感技术灵敏度高、稳定性好、检测限低等优点，以PSA为主要待测物质，其特异性抗体为分子识别元件，研究构建适用于全血或血清检测的亲和型免疫生物传感体系，为PSA的检测提供一种新的现代生物学方法。为克服常规血液检测技术中普遍存在的表面蛋白质非特异性吸附问题，本项目创新性地研究合成抑制表面蛋白质非特异性吸附的寡糖衍生物封闭剂。在传统分析方法的基础上，结合已构建的生物传感体系在实际检测中对封闭剂的特性做进一步的分析评估。并以之优化生物传感体系的性能指标，最终构建高度特异性、高灵敏度、稳定性好、检测范围宽、检测限低的PSA肿瘤标志物生物传感检测体系。本研究将进一步推进生物传感技术在医学检验领域的应用。

前列腺特异性抗原（PSA）作为前列腺癌肿瘤标志物，已被广泛应用于前列腺癌的早期诊断、预后判断、病程监测和复发监测中。本项目利用亲和型免疫生物传感技术灵敏度高、稳定性好、检测限低等优势，以PSA特异性抗体构建分子识别元件，成功构建用于全血及血清检测的传感体系。针对常规血液检测技术中普遍存在的表面蛋白质非特异性吸附问题，本项目创新性地研究合成抑制表面蛋白质非特异性吸附的寡糖衍生物封闭剂，克服了目前肿瘤标志物检测中的瓶颈。与传统分析方法结合，利用已构建的生物传感体系对实际检测中封闭剂的特性进行了深入的考察分析。通过封闭剂的性能优化，生物传感体系的特异性、灵敏度、稳定性及检测范围等分析检测性能均得到了进一步提升，实现了对PSA的高灵敏检测。本项目为PSA的检测提供了一种新的现代生物学方法，能够成为PSA临床检测的有力工具平台。