智能手机化学发光快速检测平台及其在肿瘤标志物检测中的应用研究

The rapid development of smartphones provides a new opportunity for the development of portable rapid detection devices for point-of-care testing (POCT). The routinization, low cost, rapid and sensitive detection of tumor markers is of great significance for the early discovery and early treatment of malignant tumor. This project focuses on the construction of smartphones-based chemiluminescence (CL) potable detection device for POCT and their application for tumor markers analysis. The following research work are to be carried out: (1) Fabrication of smartphones-based CL determination accessories and development of smartphones-based CL detect application to construct smartphone-based CL detection device for POCT. (2) Construction of CL analysis interface with recognition and response functions by using CL functionalized nanomaterials and specific biological identifying molecules as building blocks. Assembly of smartphone-based CL biochips based on constructed CL analysis interface. The assembly regulation, recognition and response mechanism of the system are to be investigated. (3) Application of the smartphone-based CL detection device for the simple, rapid, low-cost, sensitive or multiplex assay of biologically important tumor markers. This work is the first to develop smartphones-based CL detection platform based on CL functionalized nanomaterials and take the lead in its application for tumor markers analysis. This work is expected to enrich our knowledge on the development of portable detection devices and POCT techniques. It also expands the applications of smartphone in life-science and clinical diagnosis. Meanwhile, it will promote the practical application of advanced functional nanomaterials. This work is of great importance for early diagnosis of malignant tumor and fast detection of tumor markers.

智能手机的快速发展为开发新一代便携式快速检测设备提供了新的机遇。肿瘤标志物的低成本、快速、灵敏检测，是实现恶性肿瘤早发现、早治疗以及预后和复发监测的关键。本项目聚焦于智能手机化学发光便携式快速检测平台及其在肿瘤标志物检测中的应用研究，拟开展如下工作：（1）制备智能手机化学发光检测装置，开发智能手机化学发光检测应用程序，构建智能手机化学发光快速检测平台；（2）基于高效化学发光功能化纳米材料与识别分子的组装，构筑集识别与响应于一体的化学发光分析界面，制备智能手机化学发光检测芯片，最终实现多种肿瘤标志物的低成本、快速、灵敏检测。此项工作率先基于化学发光功能化纳米材料开展智能手机化学发光快速检测平台的设计研究，不仅丰富了便携式检测仪器和POCT分析技术的研究，拓展了智能手机在体外诊断方面的应用，而且推动了功能纳米材料的实际应用，对于恶性肿瘤的早期诊断、肿瘤标志物的快速检测等具有重要意义。

近年来，智能手机的广泛普及以及智能手机各项功能，尤其是拍照和数据处理功能的大大提升，使得智能手机成为新一代便携式检测器件的理想检测设备。肿瘤，特别是恶性肿瘤极大危害人类的健康。肿瘤的早期诊断和治疗是降低恶性肿瘤致死率的重要手段。本项目聚焦于智能手机化学发光快速检测平台及其在肿瘤标志物检测中的应用研究，开展系列研究工作。主要成果如下：（1）合成多种化学发光功能化纳米材料并积极开展相关分析应用研究工作。如将化学发光试剂鲁米诺、化学发光催化剂辣根过氧化物酶和特异性抗体共同组装到金纳米颗粒表面，合成具有特异性识别性能和优良化学发光特性的多功能化金纳米复合材料，并利用其作为化学发光分析界面，实现肿瘤标志物甲胎蛋白的无标记、高灵敏化学发光检测。（2）成功制备多通道化学发光微流控检测纸芯片，利用智能手机作为便携式检测设备，成功构建了一套智能手机化学发光快速检测平台，并进一步开发出化学发光智能手机检测应用程序，实现三种肿瘤标志物，癌胚抗原、甲胎蛋白和前列腺特异性抗的同时、快速、智能化、可视化检测。（3）此外，本课题也积极开展基于智能手机的比色型检测纸芯片分析应用研究，进一步拓展了基于智能手机的快速检测平台的研究范围。上述工作为临床上肿瘤及其他疾病标志物检测，以及便携式检测器件开发提供了有效技术手段，具有广阔的应用前景。