类受体激酶EDTS1参与调控EDR2介导抗白粉病的作用机理研究

EDR2 (ENHANCED DISEASE RESISTANCE 2) is a negative regulator in powdery mildew resistance and programmed cell death and edr2 mutants display enhanced resistance to powdery mildew (Golovinomyces cichoracearum). However, it remains unkown that how EDR2 regulats powdery mildew resistance signaling pathway. Here, we identified a mutation in edts1(edr2 suppressor 1) from the EMS mutagenesis of Arabidopsis thaliana edr2 mutants, which suppressed edr2-mediated powdery mildew resistance. The gene, EDTS1, was cloned by the map-based cloning. EDTS1 encodes a member of receptor-like kinase family and has not been reported in the plant disease resistance research. In this research, we will intensively study the function of EDTS1 in powdery mildew resistance, find the associated proteins of EDTS1, and elucidate the molecular mechanism of plant disease resistance through genetic hybridization, pathogen infection, yeast two hybrid, high performance liquid chromatography-ion trap mass spectrometry (HPLC-ITMS), surface plasmon resonance technology (SPR), co-immunoprecipitation and bimolecular fluorescence complementation. Therefore, this study is important to reveal the function of EDTS1, a novel component in the edr2-mediated disease sistance, and to elucidate the regulation mechanism of EDTS1 in the powdery mildew resistance signaling pathway.

EDR2（ENHANCED DISEASE RESISTANCE 2)是一个调控植物抗白粉病以及白粉病菌诱导产生的细胞程序性死亡的负调控因子。然而edr2介导的植物抗病信号通路的分子机制不清楚。本研究利用EMS诱变的拟南芥edr2突变体库，筛选到一个edr2的抑制子突变体edts1(edr2 suppressor 1)，并图位克隆到EDTS1基因。EDTS1编码类受体蛋白激酶家族成员，该蛋白在抗病研究中尚未见报道。本研究将采用遗传杂交、病原菌侵染、酵母双杂交、液相色谱-离子阱质谱联用仪、表面等离子共振成像技术(SPR)、免疫共沉淀和双分子荧光互补等手段，系统研究EDTS1在抗病反应中的功能，寻找EDTS1的互作蛋白及其底物。这一项目的实施，对于揭示抗病信号通路中新的组分EDTS1的功能，阐明EDTS1在抗白粉病的信号通路中的调控机理具有重要意义。

利用EMS诱变的拟南芥突变体库筛选得到一个edr2的抑制子突变体edts1,并图位克隆到EDTS1基因。EDTS1编码类受体激酶家族成员，该蛋白在抗病研究中上未见报道。本研究围绕EDTS1展开抗病机理研究，实验结果表明EDTS1影响植物对白粉菌、丁香假单胞菌、卵菌的抗性，且同时能抑制多个白粉菌抗性突变体edr2、edr1、mlo2和pmr4介导的白粉菌抗性，以及影响体内SA的积累，说明EDTS1在植物抗病过程中具有重要的调控作用。进一步利用磷酸化蛋白质谱分析技术找到EDTS1的互作蛋白FLS2及可能的靶蛋白EPP，并通过Y2H、CoIP、BiFC进行了验证，对EDTS1的作用机制进行了深入研究，该项目的实施为揭示抗病信号通路中的新组分EDTS1及其互作蛋白FLS2、EPP的功能，阐明EDTS1在抗白粉病信号通路中的调控机理具有重意义。