功能化介孔二氧化硅为载体构建的刺激响应型控制释放体系及其在肿瘤诊断中的应用

Environmental and food pollution is not only a hot issue, but also the focus of the Party Central Committee and the State Council. The number of tumor diseases caused by environmental and food pollution is increasing, which has a serious threat to human health and life safety. Therefore the early diagnosis of tumors is of great significance. Based on the innovation of the previous research, stimuli-responsive controlled release system is constructed using functionalized mesoporous silica nanoparticles as the carrier, and then the early diagnosis and prognosis of the tumor are achieved. Specifically, we design and synthesize functionalized mesoporous silica nanomaterials with good carrier properties by controlling the morphology, pore size and surface modification of the carrier. The particle size and morphology are characterized by TEM, SEM and other methods. Then, construct the controlled release system based on pH and biological molecules as stimuli with simple response mechanism and easy to implement by using polymer, organic molecule and aptamer with good seal effect as the blocking caps. Further, biocompatibility of different system is comparative studied. Finally, select good biocompatibility, low toxicity and safety controlled release system and use electrochemical, electrochemiluminescence and photoelectrochemical sensor technology platform, to achieve the goal of rapid detection of tumor markers with high sensitivity and good specificity. This project is expected to make a positive contribution to the design and preparation of mesoporous nanomaterials and the design and application of new sensing strategies.

环境和食品污染不仅是人们关心的热点问题，而且已成为党中央、国务院力求全面根治的焦点问题。由此引发的肿瘤疾病日益增多，严重威胁着人类的健康，因此肿瘤的早期诊断具有重要的意义。本项目以前期研究为创新基础，采用功能化介孔二氧化硅为载体构建刺激响应型控制释放体系，进而实现肺癌、肝癌等肿瘤的早期诊断。通过调控载体的形貌、孔径并进行表面修饰，拟设计合成具有优异载体性能的二氧化硅纳米材料，采用TEM、SEM等手段表征其粒径与形貌。然后选取封堵效果优异的聚合物、有机分子和适配体等封堵剂，构建响应机制简单、易于实现的pH和生物分子单一或双重刺激响应控制释放体系。进一步比较研究不同体系的生物相容性，最后选取生物相容性好、安全低毒的控制释放体系，利用电化学、光电及电化学发光传感技术平台，实现高灵敏、强特异性、快速检测肿瘤标志物的目标。该研究有望为介孔纳米材料的设计与制备、新型传感策略的设计与应用做出积极贡献。

环境和食品污染不仅是人们关心的热点问题，而且已成为党中央、国务院力求全面根治的焦点问题。由此引发的肿瘤疾病日益增多，严重威胁着人类的健康，因此肿瘤的早期诊断具有重要的意义。本项目以前期研究为创新基础，采用功能化介孔二氧化硅为载体构建刺激响应型控制释放体系，进而实现肿瘤的早期诊断。通过调控载体的形貌、孔径并进行表面修饰，设计合成了具有优异载体性能的二氧化硅纳米材料，通过TEM、SEM等手段表征了其粒径与形貌。然后构建了响应机制简单、易于实现的pH或生物分子刺激响应控制释放体系。结合电化学、光电及电化学发光传感技术，实现了高灵敏、强特异性、快速检测肿瘤标志物的目标。截止项目结题，课题组在Biosensors and Bioelectronics、Analytical Chemistry、Sensors and Actuators B等国际学术期刊上发表高水平SCI论文32篇，其中影响因子大于5的28篇；授权国家发明专利5件，培养研究生13名；获山东省高等学校优秀科研成果一等奖2项。该研究有望为介孔纳米材料的设计与制备、新型传感策略的设计与应用做出积极贡献。