β-TCP改性可控降解多孔镁支架复合软骨细胞体系的构建及其在软骨修复中的应用研究

The incidence of osteoarthritis (OA) is increasing in our country with ageing society. The autoallergic cartilage transplantation is one of etiological treatment of OA. However, it was limited in clinical application with inadequacy of reproductive activity of chondrocyte and support for cartilage tissue engineering. In this item, powder metallurgy and electrochemistry technique are used to constructing the degradation-controll support with porus magnesium modificated with β-TCP. The nuclear migration, cell-fusion and gene transfection are used to constructing the new chondrocyte with controllability of proliferation, SV40LTAG, HSV-TK and with out oncogenicity, The IGF1R expression vector constructed with Tet-off expression system would be transfecte in new chondrocyte, and the effect of proliferation, adhesion, migration, secretion of these cells would be investigated. The effect of construction of chondrocyte compounding degradation-controll support with porus magnesium modificated with β-TCP on repair of cartilage defect, through utilizating organismic frame in knee joint of rabbit with OA. In this item, the new chondrocyte with controllability of proliferation and construction of degradation-controll support with porus magnesium would be provided in cartilage transplantation and contributed to the etiological treatment of OA.

随着我国社会老龄化，以软骨退变损伤为特征的骨性关节炎(OA)的发生率急剧增加。自体软骨移植是OA病因治疗的手段之一，但因OA患者自身软骨细胞的增殖能力及软骨组织工程支架的缺陷而限制其临床应用。本项目采用粉末冶金法和电化学法制备β-TCP改性可控降解多孔镁支架,并检测其机械性能；应用细胞核转移、细胞融合和基因转染技术，构建含有SV40LTAG和HSV-TK、增殖可控、稳定表达IGF1R的新型软骨细胞株，鉴定其增殖、粘附、迁移、分泌等功能特性；构建新型软骨细胞-β-TCP改性可控降解多孔镁支架复合体，采用体内植入实验，明确新型软骨细胞复合多孔镁支架体系在动物体内对软骨缺损的修复作用。本项目将为镁金属医用材料复合软骨细胞应用于组织工程化软骨的研究奠定实验基础。

随着我国社会老龄化，以软骨退变损伤为特征的骨性关节炎的发生率急剧增加。本项目采用粉末冶金法和电化学法制备β-TCP改性可控降解多孔镁支架, 构建新型软骨细胞株，检测软骨细胞中与增殖、粘附、足突/细胞骨架相关的生物学行为，评价软骨细胞的功能特性；构建增殖可控的骨细胞系，基因转染，检测细胞内IGF1R、Erk1/2、JNK、AKT和OX1R的表达，检测细胞增殖功能；建立兔膝关节软骨损伤模型，观察新生软骨组织和相关蛋白表达，明确新型软骨细胞－β-TCP改性可控降解多孔镁支架复合体在动物体内对软骨缺损的修复作用。本项目为镁金属医用材料复合新型软骨细胞应用于软骨组织工程化的研究奠定实验基础。