UV-A通过JA信号途径调控大豆芽苗菜异黄酮合成的机理研究

Ultraviolet light treatment is a crucial technology for effectively improving the nutritional quality of sprouts. Our previous study found that UV-A significantly increased the isoflavonoid content of soybean sprouts, and jasmonic acid (JA) was involved in this process. However, the underlying mechanisms are still elusive. In this project, the soybean sprouts were used to explore the mechanisms of JA signaling in the biosynthesis of isoflavonoid induced by UV-A. Pharmacological experiments will be conducted to explore the relationship between endogenous JA levels and isoflavonoid content under UV-A. Transcriptome sequencing analysis will be used to identify the key genes for isoflavonoid biosynthesis and JA signaling pathway under UV-A. The Arabidopsis thaliana JA signaling transduction mutant, combined with pharmacology and molecular biology experiments, will be used to verify the function of candidate genes. The yeast two hybrid library screening, bimolecular fluorescence complementation and immune co-precipitation will be used to explore the molecular mechanism of key components of JA signaling in regulating isoflavonoid biosynthesis. The above results can not only clarify the specific mechanisms and regulation mode of UV-A regulation of isoflavonoid synthesis, deepen the scientific theory of UV-A signal regulation, but also provide useful technical information for the industrial development of edible vegetable sprouts in the future.

紫外光处理是一种可有效提高芽苗菜营养品质的关键技术。我们前期的研究发现紫外光UV-A显著提高大豆芽苗菜异黄酮含量，且茉莉酸（JA）参与UV-A诱导的异黄酮合成，但是相关的调控机理尚不明确。本课题以大豆芽苗菜为试验材料，探究JA信号在UV-A诱导大豆芽苗菜异黄酮合成中的作用机制。拟通过药理学实验探究UV-A下内源JA水平和异黄酮含量之间的关系；通过转录组测序发掘UV-A下受调控的异黄酮合成和JA信号转导关键基因；利用拟南芥茉莉酸信号转导突变体结合药理学和分子生物学实验验证关键基因的功能；通过酵母双杂交文库筛选，荧光双分子互补和免疫共沉淀等技术阐明JA信号转导关键基因调控异黄酮合成的分子机制。上述结果不仅能阐明UV-A调控异黄酮合成的具体调控模式，深化UV-A信号调控的科学理论，同时可为芽苗蔬菜设施化生产的发展提供技术依据。

紫外光处理是一种可有效提高芽苗菜营养品质的关键技术。我们前期的研究发现紫外光UV-A显著提高大豆芽苗菜的异黄酮含量，但茉莉酸（JA）在UV-A诱导的异黄酮合成的作用机制尚不明确。本课题以大豆芽苗菜为试验材料，探究JA信号在UV-A诱导大豆芽苗菜异黄酮合成中的作用机制。研究发现内源JA含量随着UV-A处理剂量的增加而增加，进一步通过转录组学测序筛选，发现JA信号通路关键因子MYC2受UV-A调控。以拟南芥MYC2缺失突变体为材料，结合UV-A处理，分析了MYC2在UV-A调控的异黄酮生物合成中的功能。研究结果表明，MYC2基因的缺失会部分程度上抑制UV-A对异黄酮合成的促进作用。利用酵母双杂交文库、BiFC试验，验证了MYC2与JAZ存在互作，这种互作可能在调控异黄酮的合成过程中起到关键作用。上述研究结果阐明了UV-A通过JA信号途径调控异黄酮合成的具体调控模式，可为芽苗蔬菜设施化生产的发展提供技术依据。