果蝇视觉学习记忆的功能神经回路解析

Drosophila is one of the important models in the study on neural circuit of learning and memory. In the central complex, F1 and F5 neurons of fan-shaped body and R2/4m neurons of ellipsoid body are all involved in vision pattern memory. Although the former dependents on figure parameters, the latter not, the fan-shaped body and ellipsoid body may participate in the learning and memory in one correlative neural circuit. With the emergence of a variety of functional imaging techniques, it is possible to resolve the complete neural circuits in Drosophila. The candidate Gal4 lines showing structural connection with F1, F5 neurons of the fan-shaped body will be screened and confirmed firstly, by blocking or activating candidate Gal4 labeled neurons are used to verify whether it involved in visual pattern memory. Using the latest functional imaging technology (CaMPARI, Synaptic-GRASP), the functional circuit between the input or output neurons and F1, F5 neurons of the fan-shaped body in visual pattern memory will be identified, and further to determine the connections with R2/4m neurons of the ellipsoid functionally. Through these studies, it will reveal the functional neural circuit basing on the fan-shaped body and ellipsoid body associated with visual pattern memory, and provide some reference for the research on the functional circuit of memory in other organisms.

果蝇是研究学习记忆神经环路的重要模型动物之一。果蝇脑内中央复合体的扇形体F1、F5神经元和椭球体R2/4m神经元均参与视觉图形记忆。虽然前者与图形参数有关，而后者与其无关，但是扇形体和椭球体可能相互关联在一个神经环路中参与学习记忆。凭借果蝇上多种功能成像新技术的出现，使解析完整神经环路成为可能。首先通过筛选和确认与扇形体F1、F5神经元可能有结构联系的候选Gal4品系，在此基础上通过阻断或活化候选Gal4所标记神经元验证其是否参与视觉图形记忆；通过采用最新功能成像技术（CaMPARI、Synaptic-GRASP等）确定在视觉图形记忆中与扇形体F1、F5神经元存在功能连接的输入或输出神经元，并进一步确定与椭球体R2/4m神经元的功能连接。通过这些研究将进一步揭示基于扇形体和椭球体神经元的视觉图形记忆功能神经环路，为其它生物记忆功能环路的研究提供一定的参考。

果蝇是研究学习记忆神经环路的重要模式动物之一。在视觉图形记忆的研究中，发现果蝇脑内中央复合体的扇形体F1、F5神经元和椭球体R2/4m神经元均参与视觉图形记忆。虽然前者与图形参数有关，而后者与其无关，但是扇形体和椭球体可能相互关联在一个神经环路中参与学习记忆。为了解决上述科学问题，我们利用果蝇中的Gal4系统和LexA系统，通过结构和功能GRASP等技术来研究扇形体与椭球体之间的结构与功能联系。首先通过组织学和行为学实验确认了扇形体F5神经元的等效Gal4品系和LexA品系，通过阻断等效Gal4品系和LexA品系影响果蝇视觉图形记忆；通过采用结构和功能GRASP成像技术，确定在视觉图形记忆中扇形体F5神经元与椭球体R2/4m神经元存在着功能连接。这些研究将进一步揭示基于扇形体和椭球体神经元的视觉图形记忆功能神经环路，为其它生物记忆功能环路的研究提供一定的参考。