RhMYB108基因参与乙烯和茉莉酸调控月季花朵衰老的机理解析

Rose is one of the most important ornamental crops around the world. The process of flower senescence is closely related to the ornamental value of rose. Previous research has shown that ethylene can accelerate the process of flower senescence which shortens the vase life. While jasmonic acid (JA) function in flower senescence is currently unclear, and the detailed mechanism by how signaling crosstalk between ethylene and JA modulate flower senescence is still poorly understood. Here, we screened one gene member of the MYB transcription factor from the transcriptome database of ethylene treatment and flower opening. This project plan to identify the function of MYB108 in process of flower senescence and to analyse the roles of important members in the signal cross between ethylene and JA by explaining the upstream and downstream regulatory networks of MYB108. The goal of this project is to provide new data for the molecular mechanism of MYB108 regulating flower senescence mediated by interplaying between ethylene and JA signaling at the transcriptional level, and to offer a new gene source for the improvement of the floral quality of rose.

月季是世界范围内重要的观赏植物，其花朵品质与衰老速度密切关联。已有研究表明植物激素乙烯能够加速花朵衰老进程，显著缩短花朵的瓶插寿命。然而茉莉酸在花朵衰老中的功能尚不明确，乙烯和茉莉酸在花朵衰老中信号互作的分子机制也是知之甚少。申请者前期工作从月季花朵响应乙烯处理的基因表达谱和花朵开放的转录组基础上，筛选获得了一个MYB家族成员MYB108。本项目拟对MYB108在月季花朵衰老中的功能进行鉴定，对其上下游的调控网络进行解析，期望从转录层面上初步阐明MYB108介导乙烯和茉莉酸信号途径，调节月季花朵衰老的分子机制，进而为月季花朵品质改良提供新的基因资源。

月季是世界范围内重要的观赏植物，其花朵品质与衰老速度密切关联。已有研究表明植物激素乙烯能够加速花朵衰老进程，显著缩短花朵的瓶插寿命。然而月季采后生产以及长距离运输过程中茉莉酸和创伤在花朵衰老中的功能尚不明确，乙烯与茉莉酸以及创伤在花朵衰老中信号互作的分子机制也是知之甚少。申请人在月季花朵响应乙烯、创伤处理和花朵发育阶段转录组数据的基础上，首先发现RhMYB108可以整合乙烯和茉莉酸信号，作为转录因子进一步靶定到衰老关联基因的启动子上激活其转录，参与月季花朵衰老进程的调控。另外发现创伤和乙烯信号在月季花朵衰老进程中具有加成效应，其中WRKY33是一个重要的调控因子，为今后月季采后品质改良提供重要的基因储备。