不同条件下低频超声治疗前列腺癌的应用基础研究

Low frequency and low intensity ultrasound combined with microbubbles has induced obviously pathology effect, and it may be a new method which has little invasiveness in the adjunctive therapy of cancer whose main biological effect is cavitation damage. However, in this research area, a crucial problem is that the experiment results can not always be repeated in the present. In all factors which influence cavitation damage, sound field is the most important and the most difficult factor which could be identified, and the detection of sound intensity of low ultrasound whose frequency is lower than common has little in the international. This subject is an extension of the past national nature science subject, the study target disease is androgen-independent stage prostate cancer, and the purpose of it is to solve difficulty of prostate cancer in clinic therapy and systematic detect main sonic parameters of low frequency ultrasound, to evaluate different frequency, and different intensity ultrasound alone or combined with microbubbles cure prostate cancer cell and transplanted tumor, to study its biological effects，to study the effects of remedial low frequency ultrasound under assistance of ultrasound image guidance on the ablation of prostate cancer, to set up ultrasound techniques platform which can be controlled and stable, choosing the best remedial parameter, and then it can assistant the ablation of prostate cancer, improve its unitary curative effect, at the same time, the platform also could be extended to study other diseases.

微泡介导的低频低功率超声辐照肿瘤细胞或增生组织已获得了明显可见的病理学结果，有望成为一种新的以非侵入性空化损伤为主要生物学效应的肿瘤辅助治疗新方法，但在该研究领域目前普遍存在实验结果重复性差的问题。在影响空化损伤的许多因素中，声功率成为最重要也最难把握的要素，而且较低频段低频超声声功率的检测目前在国际上尚处于研究的空白区。本课题是原国基金项目的延伸，以激素非依赖型前列腺癌研究为导向，解决临床前列腺癌治疗的难点为目的，系统的开展低频超声重要声学参数检测；评价不同频率、声功率低频超声单独或联合超声造影剂对前列腺癌细胞及移植瘤的生物学效应；观察治疗性低频超声在辅助超声影像引导下前列腺消融微创治疗上的作用，旨在建立可监控的、可重复性好的治疗性低频超声技术检测平台，选择优化的治疗条件，进而为辅助前列腺癌局部微创消融治疗提高整体疗效提供新的手段，同时，建立的检测技术平台对其它疾病的科学研究也将有指导意

前列腺癌是一个严重威胁老年男性的恶性肿瘤，临床治疗仍然存在难点。本研究计划从建立可控、可重复的低频超声联合微泡造影剂治疗前列腺癌的技术研发平台出发，首先在低频超声声功率的测量方面提供了一种新的测量方法；通过体内或体外实验验证了低频超声联合微泡诱导细胞凋亡、增加细胞膜通透性、促进细胞内递药、促进基因转染、阻断肿瘤及前列腺增生模型血管微循环方面的作用，为应用低频超声联合微泡治疗前列腺癌提供理论基础及研究平台。阻断肿瘤及前列腺增生模型血管微循环后进一步增加了射频消融在肿瘤及前列腺增生中的疗效，为解决临床射频消融“热沉降”效应提供了一种新的解决方法。低频超声联合微泡直接及间接促进了对前列腺癌的治疗效果，为进一步应用治疗提供了理论基础。.低频超声联合微泡治疗前列腺癌如果有效，将会对于前列腺癌的治疗具有极大的社会效益，一种新的无创的治疗方法作为现有治疗方法的一个有效补充，造福广大的患者。