基于金纳米花的表面增强拉曼生物成像新技术研究

In vivo bio-imaging technique based on surface-enhanced Raman scattering (SERS), as one of the important molecular imaging techniques, is essential for the early diagnosis of diseases and tumors. This project would start from the fabrication of the nanoparticles with abundant branches on their surfaces. This type of nanoparticles would be used as excellent SERS tags for in vivo imaging due to the plenty of 'hot spots' sitting on their surfaces. And in this SERS imaging mode, the aggregation of the nanoparticles is not required. Because of their large specific area and great light absorption properties, they could be used in the fields of drug delivery and photothermal therapy as imaging/therapy bifunctional nanotags.

利用表面增强拉曼散射（SERS）效应实现生物体的成像是分子影像技术中一种非常重要的手段,对于疾病尤其是肿瘤的早期诊断具有重要意义。本项目拟以构造表面具有支杈结构的纳米颗粒出发，以其作为SERS成像探针，借助其表面丰富的支杈所带来的数量众多的"热点"结构产生较强的表面增强拉曼活性，从而不依赖于纳米颗粒的团聚实现生物体的SERS成像；利用该种纳米颗粒较大的比表面积及其强的吸光特性，将其作为纳米药物载体或光热效应载体，实现纳米颗粒的成像-治疗双功能。

本项目以构造具有花状支杈结构的金纳米颗粒为基础，系统研究了其生理毒性，并利用其局域表面等离激元共振效应及由此产生的强的光热效应，发展了基于金纳米花的光声成像系统；同时，考虑到金纳米花颗粒尺寸较大，吸光范围较窄等缺点，我们还发展出了制备尺寸均一、具有良好的近红外光吸收特性的硫化铜量子点的新方法，对其等离激元性质进行了深入研究，发现了其等离激元共振吸收峰位置的“钉扎”现象；基于硫化铜纳米材料的等离激元和光热特性，将其与有机金属骨架（MOF）材料构造了新型光热增强的催化体系。..项目执行以来，在JACS, Chem. Sci., Nano Res.等学术期刊上发表论文6篇，培养博士研究生1名。