基于厌氧铁氧化菌的原位生物PRB对地下水砷污染的修复效应及机理研究

Hazards of arsenic contamination of groundwater and increasingly stringent standards drinking water urged to speed up the development of repair environment friendly technology. To strengthen groundwater arsenic contamination of microorganism in situ repair technology research has very important scientific significance and social value in protecting people's health and ensuring the safety of the water. Anaerobic oxidation of iron bacteria and participate in the regulation of iron oxidation process of iron and arsenic biogeochemical cycle has special significance. Screening and separating iron oxide anaerobic bacteria through the application of molecular biology in high arsenic contaminated water, the process of oxidation and migration mechanism of anaerobic oxidation of ferrous iron oxidation strain was study using SEM\EDS、XRD、HPLC-AFS、ICP technology methods, Tolerance to high concentrations of arsenic and removing mechanism are analyzed. Based on it, good biological activity and permeability PRB will be designed. Immobilized anaerobic oxidation of iron bacteria biological PRB repair technology method of arsenic contamination of groundwater will be established. This study will provide theoretical support for the anaerobic oxidation of iron bacteria on bioremediation of arsenic contamination of groundwater fields. Arsenic contamination of groundwater bioremediation technology was founded. It ensure the safety of drinking water and river water.

地下水砷污染的危害和日益严格的饮用水标准要求加速研发符合国情的环境友好修复技术，加强地下水砷污染的微生物原位修复技术研究对于保 障人民身体健康和保证用水安全具有非常重要的科学意义和社会价值。厌氧铁氧化菌及其参与调控的铁氧化过程对铁及砷的生物地球化学循环具有特殊意义。本研究通过在受污染的高砷水中应用分子生物技术筛选分离优势厌氧铁氧化菌，应用SEM\EDS、XRD、HPLC-AFS、ICP等技术方法对厌氧铁氧化菌株氧化亚铁的过程中氧化性能和铁氧化物迁移机理、菌株对高浓度砷的耐受性能和去除机理进行剖析；以此为指导，构造具有良好生物活性和渗透性能的PRB并对其影响因子进行优化，构建固定化厌氧铁氧化菌生物PRB修复砷污染地下水的技术方法，旨在为厌氧铁氧化菌在砷污染地下水场地的生物修复应用提供理论支持，为砷污染地下水规模化生物修复奠定技术基础，以保障饮用水和流域水体用水安全。

地下水砷污染的危害和日益严格的饮用水标准要求加速研发符合国情的环境友好修复技术，加强地下水砷污染的微生物原位修复技术研究对于保障人民身体健康和保证用水安全具有非常重要的科学意义和社会价值。厌氧铁氧化菌及其参与调控的铁氧化过程对铁及砷的生物地球化学循环具有特殊意义。本研究通过在受污染的高砷水中应用分子生物技术筛选分离优势厌氧铁氧化菌，对厌氧铁氧化菌株氧化亚铁的过程中氧化性能和铁氧化物迁移机理、菌株对高浓度砷的耐受性能和去除机理进行剖析；以此为指导，构造具有良好生物活性和渗透性能的PRB 并对其影响因子进行优化，构建固定化厌氧铁氧化菌生物PRB 修复砷污染地下水的技术方法，可在为厌氧铁氧化菌在砷污染地下水场地的生物修复应用提供理论支持。