木本植物原花色素分级、纳米化制备表征及抗氧化抗辐射活性相关性

Proanthocyanidins is a kind of important plant active ingredient, which has become the hot research content in plant chemistry, medicinal chemistry, forest chemistry and other the scientific fields. At present, domestic and foreign researches mainly concentrate on its antioxidant and antiradiation of the oligomeric constituents from the grape seeds and other fruit resources. Nonetheless, there are few researches focusing on antioxidant and antiradiation activity of proanthocyanidins which are derived from woody plant barks and leaves, particularly in high polymeric degree procyanins. Specially, there is almost no study focuses on the investigation of the chemical composition and antioxidant and antiradiation activity of nanoparticle proanthocyanidins. This project aims to sufficient utilize seven kinds of plant barks and leaves, such as Larix gmelinii, Pinus massoniana, Cinnamomum tree, Acacia mearnsii, Myrica rubra, Crataegus tree, and Vaccinium, which are rich in proanthocyanidins and widely used in papermaking and tannin extraction industry. Modern separation technologies will be employed to obtain high purity proanthocyanidins and other active ingredients. The chemical composition of proanthocyanidins will be analyzed by HPLC-MS, GPC and NMR methodologies. Supercritical anti-solvent process will be applied to prepare relevant proanthocyanidins. Additionally, the correlations between particle size, chemical composition and antioxidant, antiradiation activity of proanthocyanidins from bark and leaves will be investigated on the nanometer scale. In conclusion, the project will provide scientific theory and application basis to efficiently utilize the woody plant bark and leaves rooting in abundant forest resources) and to find new plant cluster possessing antoxidation and antiradiation activity. The project will promote the development of the dendro-chemistry chemical composition analysis in our country.

原花色素是一类重要的植物活性成分，对其研究已成为植物、药物和林产化学等科学领域的热点内容，但目前国内外对原花色素的抗氧化抗辐射活性研究主要针对葡萄籽等果实类资源，且主要研究低聚部分，对木本植物皮叶类来源的原花色素尤其是高聚原花色素的抗氧化抗辐射活性研究甚少，尤其是对原花色素纳米粉体的抗氧化抗辐射活性与化学组成的相关性研究尚属空白。项目针对性地选择富含原花色素的落叶松、马尾松、桂树和黑荆树、杨梅、山楂、樟树和越桔等植物皮叶，采用现代分离技术得到纯化的原花色素组分及其他活性组分，利用HPLC-MS、GPC、NMR 等分析化学组成特性，通过超临界反溶剂制备原花之间的相关性色素纳米微粒，在纳米尺度上阐明皮叶类原花色素粒径、化学组成与抗氧化抗辐射活性之间的相关性，为高效利用木本植物皮叶这一丰富的林产资源并寻找新的抗氧化抗辐射集群提供科学依据及理论和应用基础，并促进我国树木化学成分分析研究的进展。

原花色素是一类重要的植物活性成分，对其研究已成为植物、药物和林产化学等科学领域.的热点内容，但目前国内外对原花色素的抗氧化抗辐射活性研究主要针对葡萄籽等果实类资源，且主要研究低聚部分，对木本植物皮叶类来源的原花色素尤其是高聚原花色素的抗氧化抗辐射活性研究甚少，尤其是对原花色素纳米粉体的抗氧化抗辐射活性与化学组成的相关性研究尚属空白。项目针对性地选择富含原花色素的落叶松、马尾松、桂树和黑荆树、杨梅、山楂、樟树和越桔等植物皮叶，采用现代分离技术得到纯化的原花色素组分，利用HPLC-MS、GPC、NMR等分析化学组成特性，通过超临界反溶剂制备原花之间的相关性色素纳米微粒，在纳米尺度上阐明皮叶类原花色素粒径、化学组成与抗氧化抗辐射活性之间的相关性，为高效利用木本植物皮叶这一丰富的林产资源并寻找新的抗氧化抗辐射集群提供科学依据及理论和应用基础，并促进我国树木化学成分分析研究的进展。研究制备出的微粉化原花色素平均粒径在100nm左右。实验发现微粉化原花色素可明显减轻辐射对肝细胞功能的损害。应用原花色素对减轻辐射损伤动物体重丢失和血清白蛋白水平无明显作用，但微粉化原花色素效果非常显著，并且微粉化原花色素具有提高原花色素抗过氧化、免疫保护和减轻肠粘膜损伤的趋势，提示适当增加蛋白质摄入对提高机体整体抗辐射效应是有益的。