线粒体细胞色素b560的功能缺陷与衰老的分子机理研究

Two peaks of electron leak of respiratory chain has been detected by chemiluminenscence method. It was found that the first peak papear very soon after the addition of substrate, the second peak appear quite later. This property has been used for studying the mechanism of ischemia reperfusion. One patent has been applied. Experimentally proved our hypothesis of a cytochrome c mediated electron leak pathway of respiratory chain and the anti-oxidative function of cytochrome c in mitochondria , The paper was published in JBC.

细胞色素b560是线粒体呼吸链酶复合物Ⅱ的活性部位，其功能缺陷将导致多种疾病及衰老，用两种线虫突变型mev-1(短寿命)和age-1(长寿命)作为模式动物，研究其线粒体细胞色素b560功能缺陷与寿命的相关性，并同时检测活性氧、SOD和谷胱甘肽过氧化物酶的变化及抗氰粑徊浇沂救死嗨ダ系姆肿踊硖峁┲ぞ荨