基于SDF-1在神经干细胞迁移流中的时空表达探讨针刺对SAMP8小鼠神经干细胞迁移、分化的影响

The efficacy of acupuncture in the treatment of AD has been proven by clinical practice. In the related basic research, we found that the activation, migration and differentiation of endogenous NSCs regeneration is important for the repair, structure and function of AD brain lesion The Previous studies have shown that acupuncture can stimulate and regulate the proliferation and differentiation of endogenous NSCs, but the dynamic study on the migration mechanism of endogenous NSCs and the dynamic changes of NSCs differentiation in this process are not conclusive.Studied in this paper by studying mice after "the sanjiao (triple energizer) is stitch" intervention can improve rapidly aging mice behavior, and in different intervention group, different point SANP8 SVZa in mice, the RMS, OB three areas, SDF - 1 with the proliferation of neural stem cells under the action of acupuncture intervention in time and space expression in value-added, differentiation, migration, explore the SDF - 1 the expression pattern of space and time and migration of endogenous NSCs proliferation of SAMP8 acupuncture intervention brain structure reconstruction function, perfect the SDF - 1 in acupuncture treatment of AD in the process of promoting endogenous NSCs proliferation, migration has a positive effect "hypothesis.For acupuncture treatment of AD to provide an important theoretical and experimental basis.

针刺治疗AD的疗效已得到临床实践的证明，在相关基础研究中，我们发现内源性NSCs再生的激活、迁移和分化对于AD脑组织病损部位的修复、结构和功能重建，具有重要意义。前期的基础研究表明针刺能较好激发和调节内源性NSCs增殖和分化，但关于针刺促内源性NSCs迁移机制的动态研究及这一过程中NSCs分化发育的动态变化未见定论。本研究拟通过研究SAMP8小鼠经“三焦针法”干预后，可改善快速老化小鼠行为学，并且在不同干预组，不同时点SANP8小鼠脑组织中SVZa、RMS、OB 3个区域， SDF－1随针刺干预作用下增殖的神经干细胞增值、分化、迁移的时空表达情况，探究SDF－1的时空表达模式与针刺干预内源性增殖NSCs迁移对SAMP8大脑结构重建作用的相关性，完善“SDF-1在针刺治疗AD的促内源性NSCs增殖、迁移过程中具有积极影响”的假说，为针刺治疗AD进一步提供重要的理论和实验依据。

本实验基于SDF-1在NSCs迁移流中的时空表达，通过反映“迁移流”途径和方向的矢状面动态研究，探讨针刺对内源性 NSCs迁移这一神经再生修复过程必经关键环节的影响以及其动态变化，说明针刺促进内源性 NSCs 迁移和和分化作用对于神经再生修复、脑内结构和功能重建的重要意义。通过研究SAMP8小鼠经“三焦针法”干预后，观察快速老化小鼠行为学改善，检测针刺干预内源性 NSCs 增殖、并向成熟神经元、胶质细胞分化、发育的情况，以及随时间推移的动态变化，并且在不同干预组，不同时点SANP8小鼠脑组织中SVZa、RMS、OB 3个区域， SDF-1随针刺干预作用下增殖的神经干细胞增值、分化、迁移的时空表达情况，探究了SDF-1的时空表达模式与针刺干预内源性增殖NSCs迁移对SAMP8大脑结构重建作用的相关性。研究表明针刺能较好激发和调节海马和皮层内源性NSCs增殖和分化，分化形成成熟神经元，改善NSCs迁移流中的微环境。完善“SDF-1在针刺治疗AD的促内源性NSCs增殖、迁移过程中具有积极影响”的假说，为针刺治疗AD进一步提供重要的理论和实验依据。