胃俞募配穴对FD大鼠脑岛功能网络因果连接模式的调控

Combination of stomach Back-Shu and Frontal-Mu points is the classic acupuncture usage regulating stomach and the intestines. In the previous study supported by National Nature Science Fund, we found that insula played a good role in the central integration of gastrointestinal regulation effect by the grouped gastric Shu-Mu points. But, it needs further test that whether or not it is the core cerebral area for grouped gastric Shu-Mu points regulating gastrointestinal problems and treating functional dyspepsia and related causality network regulation pattern . So, this study aims, to take functional dyspepsia rat model as the carrier，with small animal functional magnetic resonance imaging techniques and conditional Granger causality analysis and gastric motility measurement，and take the functional network effective causality connectivity of insula as the focus, compare the impact on the clinic effect and related causality network regulation pattern of treating functional dyspepsia between the damaged insula and normal one. Thus, to further explore the central mechanism and contribute objective optical data to clinics by grouped gastric Shu-Mu points treating functional dyspepsia.

胃俞募配穴是针灸胃肠调节的经典用穴方案。我们前期国家自然基金项目发现，脑岛在胃俞募配穴胃肠调节中枢响应中发挥着重要作用，但其是否为胃俞募配穴调理胃肠、治疗功能性消化不良的核心脑区，及其功能网络的因果连接模式，尚有待进一步验证。因此，本课题拟以功能性消化不良模型大鼠为研究载体，综合运用经典的小动物功能神经影像技术、条件格兰杰因果分析和胃动力检测等手段，以脑岛功能网络有效因果连接为重点，对比观察脑岛损毁与否对功能性消化不良治疗效应和脑功能网络因果连接模式的影响，深入分析胃俞募配穴治疗功能性消化不良的中枢机制，为胃俞募配穴胃肠调节效应的临床运用提供科学客观的可视化依据。

研究以碘乙酰胺灌胃、郭氏夹尾配合不规则饮食复合造模的功能性消化不良（FD）大鼠为模型，以电针胃俞和中脘为治疗干预手段，运用7.0T小动物功能磁共振（fMRI）扫描和离体氢质子磁共振波谱成像（1H-MRS）等技术，通过临床效应、功能磁共振成像、离体1H-MRS检测三个方面，进一步确证了脑岛是胃俞募配穴胃肠调节效应中枢响应的核心脑区。研究结果表明：（1）电针胃俞募配穴能够改善FD大鼠一般情况，调节胃排空、胃泌素水平趋于正常；对于脑岛部分损毁FD大鼠，电针胃俞募配穴能有效地改善其一般情况，使胃泌素水平趋于正常，但对胃动力改善不明显。（2）通过小动物fMRI扫描发现，FD 模型大鼠脑岛与多个脑区的功能连接存在异常改变；电针胃俞募配穴对FD大鼠脑岛与海马、丘脑、下丘脑以及初级躯体感觉皮层功能连接度的异常改变具有一定调节作用。（3）大鼠的脑岛区域1H-MRS 检测发现，胃俞募配穴针刺对脑岛区域中Asp、Gly、NAA、Lac等代谢产物的含量有影响，且变化与体重、进食量、饮水量、胃肠动力指标改变存在一定相关性，可能是针刺通过靶向性调节脑岛功能，发挥中枢响应的关键环节之一。