雌激素受体基因多态性与山茶籽油交互作用对广西壮族妇女绝经后骨质疏松症的影响

postmenopausal osteoporosi（PMOP） is a common and frequently-occurring disease of middle-aged women, genetics, nutrition, race and age-related genetic diseases.Continuously between a variety of food nutrition and gene interactions, this "interactive" role has been to revolutionize the study of disease occurrence and development of new topics for Disease Control and Prevention. Our preliminary study results show that the Guangxi Zhuang women PMOP higher prevalence of camellia seed oil rich in phytoestrogens, the disease had better control effect. Disease genes and rich in phytoestrogens diet interaction the PMOP effects of the Guangxi Zhuang women study have not been reported. The subject of theapplication of ultrasound bone densitometer and molecular biology to detect Guangxi Zhuang postmenopausal women and estrogen receptor (ER) α and β gene polymorphisms andserum calcium, phosphorus, alkaline phosphatase and estradiol level , analysis ofpostmenopausal women with estrogen receptor α and β genotypes in Guangxi Zhuang ethnic bone mineral density and serum calcium, phosphorus, alkaline phosphataseand estradiol levels, and screening of the PMOP susceptibility genes, analysis ofsusceptibility genes of estrogen receptor α and β interaction with theconsumption of camellia seed oil on PMOP provide a theoretical basis for theeffective prevention and control disease, can greatly reduce the medical burden, but also improve the economic benefits of mountain tea seed.

绝经后骨质疏松症(PMOP)是中老年妇女常见病和多发病，与遗传、营养、种族和年龄相关的多基因疾病。食物中各种营养和基因之间持续不断地进行着交互作用，这种 “互动”作用已成为研究疾病发生、发展的新课题，给疾病防治带来一场革命。我们前期研究结果表明广西壮族妇女PMOP患病率较高，而富含植物性雌激素的山茶籽油对该病有较好的防治作用。致病基因与富含植物性雌激素饮食营养交互作用对广西壮族PMOP影响的研究未见报道。本课题应用超声骨密度仪和分子生物学技术检测广西壮族绝经后妇女骨密度和雌激素受体α和β基因多态性及血钙、磷、碱性磷酸酶和雌二醇水平，分析雌激素受体α和β基因型与广西壮族绝经后妇女骨密度及血清钙、磷、碱性磷酸酶和雌二醇水平关系，筛选PMOP易感基因，分析雌激素受体α和β易感基因与食用山茶籽油交互作用对PMOP的影响，为有效防治该病提供理论依据，可大幅度减轻医疗负担，又可提高山茶籽经济效益

我国人口老年化日益加剧，骨质疏松症逐年增多，其中女性绝经后骨质疏松症(postmenopausal osteoporosis，PMOP)发病率最高，临床症状最重。雌激素受体(Estrogen receptor, ER)基因多态性与PMOP的相关性，国内外有大量的研究，但还未得出一致结论。这可能与种族、环境、饮食营养有关。在人类基因组图谱成功绘制之后，以基因组为基础的营养学研究将给疾病治疗带来一场革命。营养和基因之间在持续不断地进行着交互作用，食物中的各种营养与体内不同类型基因之间的“互动”作用成为解决问题关键。用超声骨密度仪检测相应人群骨密度的大小。研究长期食用山茶籽油对广西壮族妇女骨密度的影响，结果是长期食用山茶籽油对广西壮族妇女PMOP有防治作用；比较广西壮族绝经后妇女正常人群和PMOP人群ERα和β基因的分布差异，分析其与广西壮族妇女的PMOP的相关性，结果P和rr基因人群患PMOP明显增多（p﹤0.05），提示P和rr基因是广西壮族妇女ERα和β基因中患绝经后骨质疏松症的易感基因；以及分析食用山茶籽油与ERα和β基因之间交互作用对广西壮族妇女绝经后骨质疏松症的影响。结果食用山茶籽油Aa基因的人群患PMOP明显减少（p﹤0.05）；提示Aa基因是食用山茶籽油与ERα和β基因之间交互作用对广西壮族妇女绝经后骨质疏松症防治作用明显的敏感基因。易感基因人群应该提前防治，敏感基因人群食用山茶籽油预防效果更好，为绝经后骨质疏松症防治的个性化和食疗提供实验依据。如果推广使用，不仅提高广西壮族绝经后妇女的健康水平，同时也可提高少数民族山区山茶籽的经济效益。成果论文6篇均发表在中文核心期刊。