应用多表位嵌合抗原监测云南省边境地区恶性疟疾传播强度

Following the decline of malaria transmission in many countries and regions, entomological estimates become less sensitive, and serological parameters become particular useful for malaria surveillance especially in low intensity areas. However, whether an appropriate serological marker can be selected is the critical core of this method. In the preliminary study, we found the multi-epitope chimeric protein, M.RCAg-1, was well-recognized by the naturally acquired anti-malaria antibodies in P. falciparum patients and had the advantage of few polymorphisms. And the preliminary study demonstrated that the M.RCAg-1 antigen had the potential as a sero-biomarker to estimate the P. falciparum malaria transmission intensity. In this project, M.RCAg-1 antigen will be used as the serological marker to estimate P. falciparum transmission intensity in the border area of Yunnan. Sero-catalytic models will be used to obtain the sero-conversion rate trough testing anti-M.RCAg-1 antibodies in the serum of local residents. This project will help to understand the epidemiological characteristics of Plasmodium malaria in border areas in Yunnan and to provide useful information for the health part to carry out the prevention and control measures.

随着大量防控措施的开展，全球疟疾死亡率和发病率都已显著下降，而且很多国家和地区已经进入疟疾消除阶段，在该阶段，加强疟疾监测并有效地评估其传播强度至关重要。由于疟疾传播强度的显著降低，传统监测指标已不再适用，而高灵敏性的血清学方法则受到越来越多的重视，该方法的关键在于选择合适的抗原标志。申请者前期研究发现人工多表位嵌合抗原（M.RCAg-1）对恶性疟疾感染后产生的天然抗体敏感性强而且人群多态性低，并初步证实了其用于评估恶性疟疾传播强度的可行性。基于前期的研究，本项目应用M.RCAg-1抗原作为检测标志物，收集云南省边境地区居民的血清，通过血清催化模型，获得当地的恶性疟疾的传播强度指标，即血清阳转率。进一步验证该抗原用于评估恶性疟疾传播强度的有效性，同时了解云南边境地区恶性疟疾的流行情况，为卫生部门开展防控措施提供有用的信息支持。