从ERK1/2和p38信号通路及其交互作用研究MEBT/MEBO促进慢性难愈合创面修复的机制

Chronic nonhealing wound, known as ulcer, has been one of the public health problems, and there is no effective treatment for the disease at the present. Studies indicated that ERK1/2 and p38 signal pathways play a key role in the repair of chronic nonhealing wound. Our previous researches proved that MEBT/MEBO effectively promote chronic nonhealing wound repair, but the mechanisms are not fully clear. Based on the previous results, the current study aims to observe the effects of MEBT/MEBO on the chronic nonhealing wound repair and cytokines using electric mirror and ELISA respectively, and to investigate the effects of MEBT/MEBO on the protein and gene expression of signal molecules such as ERK1/2, p38, MAPKK and their substrates including ATF2, MK2, c-myc, MEF2C, Akt in wounds by Western Blot and Real-time PCR respectively, which will further reveal the molecular mechanisms of MEBT/MEBO in promoting chronic nonhealing wound repair based on the signal pathways of ERK1/2 and p38 and their crosstalk, and provide the reliable theoretical basis for extensively popularizing MEBT/MEBO to treat chronic nonhealing wound in clinical practice.

慢性难愈合创面 (俗称溃疡 )已成为危害人类健康的公共卫生问题，目前临床上仍缺乏有效的治疗手段。研究表明，ERK1/2和p38信号通路在伤口愈合修复中起着关键作用。课题组前期研究表明MEBT/MEBO能有效治疗慢性难愈合创面，但机制尚未完全清楚。本项目在前期研究基础上，分别运用电镜和ELISA技术观察MEBT/MEBO对大鼠慢性难愈合创面修复和细胞因子水平的影响，以及采用Western Blot、Real-time PCR探讨MEBT/MEBO对慢性难愈合创面信号分子ERK1/2、p38、MAPKK及其底物ATF2、MK2、c-myc、MEF2C、Akt等蛋白和基因表达的影响，以期从ERK1/2和p38信号通路及其交互作用(crosstalk)角度进一步阐明MEBT/MEBO促进慢性难愈合创面修复的分子机制，为临床推广应用MEBT/MEBO防治慢性难愈合创面提供可靠的理论依据。

慢性难愈合创面(俗称溃疡)已成为危害人类健康的公共卫生问题，目前临床上仍缺乏有效的治疗手段。研究表明，ERK1/2和p38信号通路在伤口愈合修复中起着关键作用。课题组前期研究表明MEBT/MEBO能有效治疗慢性难愈合创面，但机制尚未完全清楚。本项目在前期研究基础上，分别运用电镜和ELISA技术观察MEBT/MEBO对大鼠慢性难愈合创面修复和细胞因子水平的影响，以及采用Western Blot、Real-time PCR探讨MEBT/MEBO对慢性难愈合创面信号分子ERK1/2、p38、MAPKK及其底物ATF2、MK2、c-myc、MEF2C、Akt等蛋白和基因表达的影响，以期从ERK1/2和p38信号通路及其交互作用(crosstalk)角度进一步阐明MEBT/MEBO促进慢性难愈合创面修复的分子机制，为临床推广应用MEBT/MEBO防治慢性难愈合创面提供可靠的理论依据。