MEBT/MEBO对慢性难愈合创面修复中表皮细胞行为调控机制研究

The chronic wounds surface is a common clinical disease with complex pathogenesis. Due to its intractability in clinical treatment, the research of wound repair mechanism becomes a hot spot as well as a difficult issue.. The traditional Chinese medicine has been used efficiently in the treatment of the chronic wounds surface for a long history. Moist Exposed Burn Therapy/Moist Exposed Burn Ointment (MEBT/MEBO) originated from the therapy of saprophytic tissues removal in traditional Chinese medicine . Our group in preliminary study found that MEBT/MEBO can obviously improve the rat skin cell ultrastructure, stablize internal environment in cells, and facilitate the proliferation differentiation of bFGF and VEGF, thus accelerating the reepithelial and the reshaping of the organizational structure, but the mechanism is not fully clear yet.. A hypothesis proposed by our group is as follows : MEBT/MEBO can realize its role for wound repair in the treatment of the chronic wound by means of regulating skin cell behavior. To verify this hypothesis , our plan is to establish the rat model with chronic wounds difficult to heal, through experiments in vivo and in vitro, applying the technology interference to the wound, and using immunofluorescence staining, confocal laser scanning and Real - time qPCR and Western blot detection technology, so as to reveal MEBT/MEBO’ regulatory mechanism for biological behavior like epidermal cells proliferation , mitosis and differentiation during the process of chronic wound repair, which can provide convincing theoretical basis for clinical application of this technique for prevention and cure of chronic wounds surface.

体表慢性难愈合创面是临床常见病症，其发病机制复杂，临床治疗较为棘手，因而创面修复机制探讨成了当今研究的热点和难点。.中医药治疗皮肤溃疡历史悠久，效果明显。皮肤再生医疗技术(MEBT/MEBO)源于中医祛腐生肌法，课题组在前期研究中发现MEBT/MEBO有改善大鼠表皮创面细胞超微结构、稳定细胞内环境、增快bFGF及VEGF增殖分化的作用，从而加速创面再上皮化和组织结构的重塑，但机制尚未完全清楚。.课题组提出假说：MEBT/MEBO可能对创面修复过程中表皮细胞行为的调控起作用。拟建立慢性难愈合创面大鼠模型，通过体内外实验，应用该技术对创面的干预，并运用免疫荧光染色、激光共聚焦断层扫描及Real-time qPCR和Western blot等检测技术，揭示MEBT/MEBO在创面修复过程中对表皮细胞増殖、分化和有丝分裂等生物行为的调控机制，为临床应用该技术防治慢性难愈合创面提供更有力的理论依据。

体表慢性难愈合创面是临床常见病症，其发病机制复杂，临床治疗较为棘手，因而创面修复机制探讨成了当今研究的热点和难点。中医药治疗皮肤溃疡历史悠久，效果明显。皮肤再生医疗技术（MEBT/MEBO）源于中医祛腐生肌法，课题组通过建立慢性难愈合创面大鼠模型，通过体内外实验，应用该技术对创面的干预，并运用免疫荧光染色、Real-time qPCR和Western blot等检测技术检测大鼠慢性难愈合创面基底膜中CK10、CK14、CK15、CK19、PTEN、p-Akt等的表达情况，表皮细胞体外模型CK10、CK14的mRNA转录水平和蛋白表达。实验结果表明MEBT/MEBO能够促进慢性难愈合创面愈合，且能调控创面组织中细胞角蛋白的表达水平。进一步揭示MEBT/MEBO可以改变细胞外环境，调控创面肉芽组织中CK10、CK14、CK15、CK19等蛋白的表达以及mRNA的转录水平，从而促进表皮干细胞的增值与分化，加速慢性难愈合创面的修复。为临床应用该技术防治慢性难愈合创面提供更有力的理论依据。