肥大细胞亚型鉴定和激活特征

Using newly developed monoclonal antibody in the current study, we demonstrated that the majority of mast cells in synovium are carboxypeptidase positive. The numbers of these cells in the synovial tissue of osteoarthritis were increased dramatically indicating that they are highly likely to play a key role in the pathogenesis of this disease. The differences between the actions of lung and skin tryptases in induction of inflammatory cell accumulation suggested that subtypes of tryptases from these two sources could be different. The selective inhibition of enzymatic activities of tryptase and chymase by natural and synthetic protease inhibitors added novel information in the drug development process in mast cell research area. Stimulation of tryptase release from mast cells by tryptase receptor agonists indicated that tryptase can stimulate mast cells in a positive feedback way. These findings supplied some novel information on the understanding of mast cell associated diseases.

应用不同标记的抗人类肥大细胞类胰蛋白酶和类糜蛋白酶抗体及免疫组织化学双重标记技术，对正常组织和病变组织中的肥大细胞进行染色，从而确定肥大细胞的亚型分类、比例和在疾病中的变化，为肥大细胞领域增加新的内容，用抗IgE等对酶悬浮和纯化的肥大细胞及其亚型进行刺激，从而查出各种亚型肥大细胞对刺激的反应性，添补这方面理论的空白。.