Raf36介导拟南芥感染寄生疫霉菌的机制研究

Phytophthora, a genus of oomycetes, causes destructive diseases on plants. Understanding the mechanism of plant-Phytophthora interactions is crucial for developing new strategies to control Phytophthora diseases. Compared to extensive exploration of incompatible interactions between plants and oomycetes, knowledge about plant-Phytophthora compatible interactions remains obscure. Our previous researches identified a MAPKKK gene Raf36 that negatively regulates resistance of Arabidopsis thaliana to Phytophthora parasitica and suppresses hypersensitive response (HR) triggered by Avrblb1/Rpi-blb1 gene pair in Nicotiana benthamiana. In this project, we aim at seeking correlated genes involved in Raf36 mediated HR-suppression, analyzing re-localization of Raf36 at subcellular level, and explicating the mechanism of Raf36 to interfere SA signaling pathway. In summary, clarifying the role of Raf36 in impairment of ETI is anticipated, which will aid to create new strategies to control Phytophthora diseases.

疫霉菌是卵菌界中一类重要的植物病原菌，掌握植物对疫霉菌的感病机制是有效防控病害的前提。基于拟南芥-寄生疫霉菌亲和互作体系，课题组前期研究发现Raf36基因负调控拟南芥对寄生疫霉菌的抗性，且抑制由马铃薯晚疫病菌效应子Avrblb1与马铃薯晚疫病抗病蛋白Rpi-blb1识别引起的过敏性坏死反应。本项目拟借助模式卵菌病害体系，利用突变体和转基因植株的转录组测序分析，寻找Raf36功能相关基因，并初步分析其功能；通过免疫标记确定Raf36的功能基序和亚细胞定位基序的联系；基于SA（水杨酸）抗病途径标记基因的表达模式分析，结合不同转基因植株中SA表达量的差异，明确Raf36抑制SA信号通路的机理。综合以上结果，期望阐明疫霉病菌挟持植物自身基因改变植物抗/感病性的机制，为作物卵菌病害新型防治策略的研发提供理论依据。