新型抗菌生物活性高分子盖髓材料在活髓保存中的效应研究

The success rate of vital pulp preservation in the treatment of deep caries is low, and the existence of bacteria is the most important factor. The low success rate has aroused the concern of scholars of stomatology. The main shortcoming of pulp capping materials commonly used in clinical is lack of good antibacterial property. In this study, a novel antibacterial bioactive pulp capping polymer material will be developed, which contains both quaternary ammonium salts antibacterial monomer MAE-DB and bioactive calcium-silicate. The best composition ratio of the novel antibacterial bioactive pulp capping polymer material will be determined by optimizing the copolymerization conditions and by evaluating the antibacterial property, physical and chemical properties. The biological activity of the novel pulp capping polymer material will be evaluated in the aspects of promotion of the pulp recovery, induction of dentin remineralization and promotion of dentin bridge formation by in vitro, in vivo and cytology experiments. Completion of this study will endow pulp capping materials with excellent antibacterial properties, and will provide solutions to improve the low success rate of vital pulp preservation in the treatment of deep caries.

细菌感染是导致深龋活髓保存成功率低的主要原因，也是口腔医学界关注的难点问题，缺乏优良的抗菌性能是目前临床常用盖髓材料存在的瓶颈问题。对此，本课题组提出了将前期合成的具有较强抗菌活性的可聚合季铵盐抗菌单体MAE-DB与具有明确诱导牙本质再矿化作用的活性硅酸钙共同引入树脂体系中的方案，以形成兼具优良抗菌性能及诱导牙本质再矿化性能的高分子盖髓材料。基于此方案，本研究拟通过共聚工艺配方优化、抗菌性能及理化性能评价，确定新型抗菌生物活性高分子盖髓材料各组份的最适配比；进而通过体外实验、动物体内实验、细胞学实验全面探讨新型抗菌高分子盖髓材料的生物活性，评价其在促进牙髓组织恢复、诱导牙本质再矿化、促进牙本质桥形成等方面的效应。本项目的完成将为赋予盖髓材料优良的抗菌性能提供新的思路，为提高深龋治疗过程中活髓保存的成功率提供实验依据。

细菌感染是深龋活髓保存治疗失败的主要原因之一，而目前临床常用的盖髓材料抗菌性能均达不到理想水平。对此，本课题组将具有较强抗菌活性的可聚合季铵盐抗菌单体MAE-DB与具有明确诱导牙本质再矿化作用的活性硅酸钙共同引入树脂体系中，合成了一种新型的抗菌盖髓材料。本课题所研发的填料与树脂比例为2:1及3:1的两组实验盖髓材料填料分布均匀，具有稳定持久的接触抗菌性能，良好的生物安全性，适合的固化深度、双键转化率、吸水率和溶解度，持续的钙离子及羟基离子释放能力，良好的体外生物活性及体内盖髓效果，有望作为理想的盖髓材料用于临床治疗。