生物可降解镁合金支架治疗动脉再狭窄的实验研究

Peripheral artery disease secondary to diabetes remains a major problem and might require stenting therapy. However, the major problem of the traditional mental stents is its in-compatibility with patient tissues and thrombosis which have a huge impact on its acceptability. Compared with traditional mental stents, biodegradable stents could degrade, reduce the recurrence of stenosis and allow repeated surgical operation at the occlusion sites without being permanently remained in the bodies. We have developed a patented biodegradable magnesium alloy (Mg-Sn-Mn). The preliminary results revealed that this magnesium alloy was better than the current stent materials. However, the biocompatibility, biodegradability, mechanical properties and safety of this biodegradable magnesium-alloy needs to be further studied. The present study aims to investigate the biocompatibility, biodegradability and mechanical properties of this biodegradable magnesium-alloy and its therapeutic potential on stenosis in tissue culture and canine iliac artery in-stent restenosis model. This study would help us to understand the signalling pathways of cell apoptosis, proliferation and inflammation involved in this biodegradable magnesium-alloy stent, thereby providing the experimental basis for the clinical application of magnesium-alloy stents.

糖尿病等引起的下肢动脉硬化闭塞症在临床十分常见，危害很大，常需进行血管支架治疗。传统的金属支架易形成血栓且组织相容性欠佳，严重影响疗效，患者不愿接受。与金属支架相比，生物可降解支架可自行降解，减少血管再狭窄，并可在血管闭塞处反复手术操作。我们前期开发的专利产品--新型生物可降解Mg-Sn-Mn系镁合金支架，初步试验表明优于现有的支架材料；但是，其生物相容性、理化性能、安全性和防治机制需进一步研究。我们拟复制犬髂动脉狭窄和细胞培养模型，采取功能和形态相结合的方式，观察我们上述研制的支架、不锈钢传统支架及其他镁合金支架对动脉狭窄的防治效果和再狭窄情况，从整体、组织、细胞和分子水平，探讨这种新型生物可降解Mg-Sn-Mn系镁合金支架在体内外的生物相容性和生物理化性能特点；从细胞凋亡、增殖、炎症及其信号传导途径的角度，揭示其防治动脉再狭窄的机制，为其在临床的推广应用提供理论基础和实验依据。

高血压、糖尿病等引起的下肢动脉硬化闭塞症在临床十分常见，危害很大，常需进行血管支架治疗。传统的金属支架易形成血栓且组织相容性欠佳，严重影响疗效，患者不愿接受。与金属支架相比，生物可降解支架可自行降解，减少血管再狭窄，并可在血管闭塞处反复手术操作。我们前期开发的专利产品——新型生物可降解Mg-Sn-Mn系镁合金支架，初步试验表明优于现有的支架材料；其生物相容性、理化性能、安全性和防治机制在本项目中得到进一步证实。我们复制犬髂动脉狭窄和细胞培养模型，采取功能和形态相结合的方式，观察我们上述研制的支架、不锈钢传统支架及其他镁合金支架对动脉狭窄的防治效果和再狭窄情况，从整体、组织、细胞和分子水平，探讨了新型生物可降解Mg-Sn-Mn系镁合金支架在体内的生物相容性和生物理化性能特点；从细胞凋亡、增殖、炎症及其信号传导途径的角度，揭示其防治动脉再狭窄的机制，为其在临床的推广应用提供理论基础和实验依据。