食品级纳米运载体系增强酚类物质生物利用度的机理研究

Nano delivery systems have potential advantages in improving the oral bioavailability of phenolics, which would lead to the enhancement of their bioactivities in human bodies. However, these days, most of the researches of nano delivery systems in food science area were mainly focus on the rational design of nano delivery systems and their digestion and release in the gastrointestinal tract. The mechanism lying behind the effects of nano delivery systems on the absorption, transportation and distribution of phenolics is still unknown. Therefore, in this project, we will study the relationship between the digestion, release and absorption of phenolics in the gastrointestinal tract and the structures and compositions of nano delivery systems by using in vitro digestion model combined with Caco-2 cell monolayer model. Moreover, we will also investigate the impacts of nano delivery systems on the in vivo transportation, distribution and metabolism of phenolics by using Sprague-Dawley Rat model. Finally, the mechanism of the enhancing effects of nano delivery systems on the oral bioavailability of phenolics will be summarized. This study will have great implications for the future application of phenolics in the food industry.

纳米运载体系对改善酚类物质的口服生物利用度有着巨大潜力，能够促进其生物活性的有效发挥。然而现阶段食品领域酚类物质纳米运载体系的研究主要集中在纳米运载体系的设计与构建及初步的体内释放效果研究方面，纳米运载体系调控酚类物质肠内吸收及体内转运分布的内在机理尚不明确。因此，本项目拟构建不同结构、成分的酚类物质纳米运载体系，采用体外消化模型结合Caco-2单层细胞模型，利用生物物理手段深入研究酚类物质在消化系统中的消化释放效果及肠内吸收转运机制与纳米运载体系成分、结构间的关联性，并利用SD大鼠模型探索纳米运载体系对酚类物质体内转运分布代谢的影响，揭示纳米运载体系增强酚类物质口服生物利用度的内在机理，为促进食品中酚类物质体内生物活性的有效发挥提供了重要的理论依据和指导，进而实现酚类物质在食品工业中的科学、高效利用。

纳米运载体系对改善酚类物质的口服生物利用度有着巨大潜力，能够促进其生物活性的有效发挥。然而现阶段食品领域酚类物质纳米运载体系的研究主要集中在纳米运载体系的设计与构建及初步的体内释放效果研究方面，纳米运载体系调控酚类物质肠内吸收及体内转运分布的内在机理尚不明确。.本项目采用变性淀粉、大豆蛋白、乳清蛋白等多种材料分别构建纳米乳液、脂质体、W/O/W多层包埋体系等多种不同结构类型的酚类物质纳米运载体系，并采用体外消化模型结合Caco-2单层细胞模型，利用生物物理手段深入研究酚类物质在消化系统中的消化释放效果及肠内吸收转运机制与纳米运载体系成分、结构间的关联性，研究结果表明壳聚糖修饰能够有效地提升脂质体结构的缓释效果，并能够在一定程度上提升包埋在运载体系中的白藜芦醇小肠上皮细胞的吸收效果，相关研究在《Journal of Controlled Release》(IF: 7.901)等期刊杂志上发表。最后，采用SD大鼠模型探索纳米运载体系对酚类物质体内转运分布代谢的影响，意图揭示纳米运载体系增强酚类物质口服生物利用度的内在机理，为促进食品中酚类物质体内生物活性的有效发挥提供了重要的理论依据和指导，进而实现酚类物质在食品工业中的科学、高效利用。