Her-2靶向导入miRNA治疗乳腺癌研究
基本信息
结项摘要
microRNA（miRNA）能够抑制致病基因转录表达，是潜在的治疗恶性肿瘤的基因武器。如何在体内把miRNA定向导入目标细胞是应用miRNA治疗恶性肿瘤的关键问题。本课题拟结合具有细胞导向功能的单链片段抗体（ScFv）技术和RNAi技术，研制抗Her2受体的ScFv与荷正电多肽的融合蛋白，以携带抗肿瘤miRNA表达载体选择性导入Her2阳性的乳腺癌细胞。同时，用Her2 ScFv修饰能浓缩基因物质的脂质体，制成具有定向结合Her2受体和携带siRNA双向功能的免疫脂质载体。分别在体外乳腺癌细胞培养，以及荷瘤鼠和肝转移模型中检测上述非病毒载体导向工具输送miRNA表达载体的功能和抗瘤效应。开发基于导向性miRNA 的新型基因抗癌药物，同时为miRNA靶向导入目标细胞的研发提供新思路。
项目摘要
项目成果
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数据更新时间：2023-05-31
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