NGF相关信号通路在眼表鳞状上皮化生中的功能研究

Squamous metaplasia is a pathologic process that frequently occurs in nonkeratinized stratified ocular surface epithelia, characterized with hyperproliferation and abnormal differentiation. Our preliminary studies showed that NGF signaling pathway related with squamous metaplasia of some ocular surface deseases. To investigate the molecular mechanism of NGF signaling pathway in squamous metaplasia, the project will carried out as follow. 1)Expression changes of genes of NGF signaling pathway in clinical samples of ocular surface deseases. 2)Dynamic changes of genes of NGF signaling pathway in model of squamous metaplasia in vitro or in vivo. 3)Effects of NGF signaling pathway on the phenotype and physical function of ocular surface tissues. 4)Therapeutic effects of NGF on ocular surface deseases of samous metaplasia. This project is conducive to clarify the mechanism of Squamous metaplasia, and to provide new therapeutic methods of the desease.

鳞状上皮化生是多种眼表疾病共有的病理改变，主要表现为上皮细胞的高度增殖和异常分化。我们前期研究发现，NGF相关信号通路与部分眼表鳞状化生类疾病存在着密切关联。为了深入探究NGF相关信号通路影响眼表鳞状化生类疾病的分子机理，本项目将进行如下研究(一)临床眼表鳞状上皮化生类病变组织中NGF信号通路相关基因的表达变化；(二)体外和体内眼表鳞状化生模型中NGF信号通路相关基因的动态变化；(三) NGF相关信号通路活性改变对眼表组织表型及生理功能的影响；(四)NGF对眼表鳞状上皮化生类眼表疾病的治疗效果。本研究的开展有利于阐明眼表鳞状上皮化生的分子机理，也将为该种疾病的治疗提供新的思路和方法。

为了深入探究NGF相关信号通路影响眼表鳞状化生类疾病的分子机理，本项目进行了如下研究(一)临床眼表鳞状上皮化生类病变组织中NGF信号通路相关基因的表达变化；(二)体外和体内眼表鳞状化生模型中NGF信号通路相关基因的动态变化；(三) NGF相关信号通路活性改变对眼表组织表型及生理功能的影响；(四)NGF对眼表鳞状上皮化生类眼表疾病的治疗效果。本项目研究发现了多条与NGF作用相关的信号通路，并深入阐明了NGF抗眼表鳞状上皮化生的分子机理，为该种疾病的治疗提供新的思路和方法。