利用斑马鱼模型研究三氯生的甲状腺激素干扰效应及机制

Triclosan (TCS) is an antimicrobial additive in many personal care and household products, and evidence indicates that it can be thyroid hormone disrupting. The related research mainly focuses on amphibians and mammals, while TCS effects on fish thyroid axis have not been investigated. Thyroid hormones regulated a wide range of biological processes associated with development, metabolism, energy provision and reproduction invertebrates. Zebrafish embryos have been shown to be a useful model for investigating endocrine disruption. Since zebrafish embryo development is very similar to embryogenesis in higher vertebrates including humans, this species is ideally suited to study the thyroid function underlying embryonic development. The study focuses on the effects of TCS exposure on thyroid hormones levels and embryo development. The expression of related genes in the hypothalamic–pituitary–thyroid (HPT) axis and quantitative analysis of the T4 immunofluorescence signal were evaluated to explain the mechanisms of thyroid disruption by TCS .Zebrafish embryos were further exposed to environmentally relevant concentrations of the TCS for 5 months to characterize the disrupting effects of long term exposure on the thyroid endocrine system in adult fish and their progeny following parental exposure to TCS.

三氯生(TCS)作为杀菌剂被广泛的添加到个人护理品中，它的长期使用所带来的环境问题及其安全性评估是目前研究的热点。已有报道表明三氯生具有甲状腺激素干扰效应，但目前的研究主要集中在两栖动物和哺乳动物，对鱼类的研究不足且以成鱼为主，对鱼类早期发育阶段甲状腺激素干扰效应研究缺乏，对三氯生长期低剂量长期暴露对鱼类全生命周期的风险评估滞后。本课题正是针对目前的研究现状，以斑马鱼为模型，研究在鱼类早期发育阶段三氯生的甲状腺干扰效应和机制，同时进一步开展环境相关剂量三氯生长期暴露对斑马鱼亲代和子代的甲状腺干扰效应研究，探讨三氯生对亲代胚胎-仔鱼（F0）、亲代成鱼(F0)和子代胚胎-仔鱼(F1)的甲状腺激素干扰效应，全面评估三氯生对水生生物的安全，阐明其甲状腺干扰效应及机制，为TCS的使用和管理提供科学依据。