色氨酸分解代谢调控Treg/Th17分化拮抗哮喘中性粒细胞性炎症的作用及机制研究

Neutrophils asthma is still a lack of effective treatment, Th17 cells is closely related to the pathogenesis of asthma neutrophil cells. IDO vivo secretory capacity DC (IDO+DC) through IDO metabolism of tryptophan regulatory T cell differentiation, is considered to be the conversion of Th17 to Treg molecule "switch." Combined with our previous work: tryptophan metabolites in immunotherapy procedure helps correct Treg/Th17 imbalance in asthma research group speculated that neutrophils may lack IDO+DC subsets, immune responses, you can not differentiate the regulation of Th17 to Treg and disease. Based on this, the project intends to ① confirmed Th17/Treg imbalance involved in neutrophil asthma. ② confirm the regulation of tryptophan metabolites Th17/Treg differentiation involved in neutrophil asthma and its mechanism. ③ flow cytometry sorting health model in vivo IDO+DC, through in vitro, in vivo tests confirmed IDO+DC preventive effect on neutrophil asthma. This paper aims to investigate the pathogenesis of asthma, neutrophils, to manipulate the expression of IDO in vivo DC asthma prevention neutrophils provide a theoretical basis.

中性粒细胞哮喘目前仍缺乏有效治疗手段，研究证实Th17细胞与其发病密切相关。体内具有分泌IDO能力的DC(IDO+DC)可通过IDO代谢色氨酸调节T细胞分化，被认为是Th17向Treg转换的分子“开关”。结合我们前期工作：色氨酸代谢产物在免疫治疗过程有助于纠正Treg/Th17失衡，课题组推测中性粒细胞哮喘可能缺乏IDO+DC亚群，发生免疫应答时，不能调控Th17向Treg分化而发病。基于此，本项目拟①证实Th17/Treg失衡参与了中性粒细胞哮喘。②证实色氨酸代谢产物调控Th17/Treg分化参与了中性粒细胞哮喘并探讨其机制。③流式细胞技术分选健康模型体内IDO+DC，通过离体、在体试验证实IDO+DC对中性粒细胞哮喘的预防作用。本课题旨在探讨中性粒细胞哮喘发病机制，为操控体内DC表达IDO防治中性粒细胞哮喘提供理论依据。

中性粒细胞哮喘目前仍缺乏有效治疗手段，研究证实Th17细胞与其发病密切相关。体内具有分泌IDO能力的DC(IDO+DC)可通过IDO代谢色氨酸调节T细胞分化，被认为是Th17向Treg转换 的分子“开关”。结合我们前期工作：色氨酸代谢产物在免疫治疗过程有助于纠正Treg/Th17 失衡，课题组推测中性粒细胞哮喘可能缺乏IDO+DC亚群，发生免疫应答时，不能调控Th17向Tr eg分化而发病。基于此，本项目拟①证实Th17/Treg失衡参与了中性粒细胞哮喘。②证实色氨 酸代谢产物调控Th17/Treg分化参与了中性粒细胞哮喘并探讨其机制。③流式细胞技术分选健 康模型体内IDO+DC，通过离体、在体试验证实IDO+DC对中性粒细胞哮喘的预防作用。本课题旨 在探讨中性粒细胞哮喘发病机制，为操控体内DC表达IDO防治中性粒细胞哮喘提供理论依据。