适于在酵母中表达的高活力α- 淀粉酶基因的筛选
基本信息
结项摘要
alpha-amylase gene from Bacillus subtilis HN 503 ,from Xanthomonas campestuis pv. Campestris and from Aspergillus oryzae HN 504 were cloned.Mutator whose alpha-amylase activity and thermostability can be greatly improved was obtained by error-prone PCR. We try to clone alpha-amylase (amyA) gene from Xanthomonas campestuis into Pichia pastoris GS115 to express and secret. Recombinant GSXA was cultured in flask, and the enzyme activity of supertanant reach 33.70u/ml.In this reseach,we first apply LBSP (LB medium with trypan blue) to clone amyA gene and to screen recombinants. An alpha-amylase gene with high enzyme activity was obtained successfully by replacing dNTP with BrdUTP during PCR.
从三个供试芽胞杆菌中克隆异源a-淀粉酶基因并采用模拟分子进化的DNAshuff1ing技术筛选能大幅度提高其酶活性和热稳定性的突变基因，
项目摘要
项目成果
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暂无此项成果
数据更新时间：2023-05-31
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