鱼腥草中甲基正壬酮“节点”代谢网络的研究

On the basis of the former youth fund project, and as only detection and control one or two plant medical ingredients called "dot" control mode in Chinese medicine field from western medicine system, the project makes research on the traditional Chinese medicine plant metabolism network, which aims to explore a new, suitable for Chinese medicine field "face" control mode. The Project acts herba Houttuyniae as model, methyl-n-nonylketone as metabolic "node",we established the methyl-n-nonylketone "node" metabolic network mathematics model. And then the affinity chromatography principle "fish "for "the father and son metabolic enzymes" of 2-undecanone; To simulate plant metabolism environment will be put into the metabolic enzymes water extraction system and naphtha component system components, detection of the concentration of the components change, root out the 2-undecanone "peripheral" metabolic component. Finally to combine the constituent of varying concentrations of value and the established mathematical model, yield the balance constant between the ingredients (quality effect coefficient); Comprehensive results rendering the methyl-n-nonylketone "node" metabolic network, thus further will get the traditional Chinese medicine with parent nuclear component system of metabolic "LAN", based on the study of plant metabolic pathway, finally realizes the traditional Chinese medicine plant metabolism network construction. At last realizes to span from "point" mode to "face" quality control mode in the traditional Chinese medicine field.

本项目在前青年基金项目的基础上，针对目前中药领域大多仍借鉴采用西药的只检控一两种中药成分的“点”质制模式，展开对中药植物代谢网络研究，旨在探索一种新的、适宜中药领域的“面”质控模式。项目中拟以鱼腥草为模型中药，以甲基正壬酮为代谢“节点”，首先建立甲基正壬酮“节点”代谢网络数学模型；然后利用亲和色谱原理“钓取”甲基正壬酮的“父子代谢酶”；再模拟植物体内代谢环境将代谢酶置入其水提成分体系和挥发油成分体系，检测各成分浓度的变化，揪出甲基正壬酮的“周边”代谢成分；最后将各成分的浓度变化值与所建立的数学模型相结合，得出各成分间的平衡常数（质量作用系数）；综合结果绘制出甲基正壬酮“节点”代谢网络，继而进一步描绘得到中药同母核成分体系的代谢“局域网”，结合目前的植物代谢途径，最终实现中药植物代谢网络的构建，为实现中药领域的“点”质控模式向“面”质控模式跨越奠定基础。

本项目是青年-面上连续资助项目，是继“运用信息熵原理控制鱼腥草中药材质量稳定性研究”之后研究中药鱼腥草原植物体内甲基正壬酮的“节点”生源次级代谢网络（途径）。本项目展开了对中药原植物中生源次级代谢网络的研究，旨在探索一种新的适宜中药质量研究复杂性的质控方法与体系，进一步为中药的质量研究奠定基础。项目中以鱼腥草原植物为模型对象，以甲基正壬酮为一个生源代谢“节点”，首先建立了甲基正壬酮次级代谢“节点”网络数学模型；然后制备甲基正壬酮亲和色谱介质，再利用亲和色谱原理“钓取”甲基正壬酮相关的“父子代谢酶”；再模拟鱼腥草植物体内次级代谢环境将代谢酶置入其水提成分体系和挥发油成分体系，通过检测各成分浓度的变化，揪出了甲基正壬酮的“周边”各代谢成分；最后将其所揪出来的各代谢成分的浓度变化值与所建立的数学模型相结合，测算出了各成分间的平衡常数（质量作用系数）；综合以上结果绘制出了鱼腥草中甲基正壬酮次级代谢“节点”网络。结合目前已有的植物初级代谢途径，最终勾勒出中药植物体内次级代谢途径或网络，为实现中药质量研究的新模式奠定了基础。迄今项目组成员已即将获得国家发明专利2项、发表科研论文9篇，参加学术交流10余次，后续还将会有成果产生。