长链非编码RNA-BC027988在肝癌中的功能及其分子机制研究

Long noncoding RNAs (lncRNAs) is a group of RNAs, which are longer than 200 nucleotides without containing functional open reading frames and can't encode proteins. Recently, dysregulated expressions of lncRNAs have been reported in many types of cancers, indicating that they maybe play critical roles in tumorigenesis. However, the role of lncRNAs to hepatocellular carcinoma (HCC) remain largely unknown, the molecular mechanisms of their functions are still lacking. In this study, we have identified differentially expressed lncRNAs between HCC and paired peritumoral tissues by lncRNA/mRNA microarray. We have built coding-noncoding gene co-expression network to identify interactions among lncRNAs in cancer tissues. Furthermore, we have find the corresponding mRNAs and developed the GO and pathway annotation in order to predict the function of lncRNAs. To validate microarray analysis findings, we randomly selected lncRNAs among the differential lncRNAs and analyzed their expression using qRT-PCR in 58 pairs of HCC and corresponding nontumor liver tissues. The results confirmed that the LncRNA- BC027988 was up-regulated in HCC tissues. Meanwhile, BC027988 can promote cell proliferation and migration in vitro. In this study, we will focus on the expression levels and Clinical Significance of BC027988 in HCC as well as its functions and undelying molecular mechanism to provide new insights into genetic diagnosis and treatment of hepatic carcinoma in clinic.

长链非编码RNA（LncRNA）是一组长度大于200个核苷酸、缺少特异完整的开放阅读框、无蛋白质编码功能的RNA。研究发现，LncRNA的表达差异能影响下游基因的表达，从而参与肿瘤的发生发展，但其在肝癌中的作用及其分子机制仍不清楚。本研究结合LncRNA/mRNA表达谱芯片和生物信息学分析，筛选出肝癌中差异表达的LncRNA并构建共表达网络，从已知功能的mRNA推测LncRNA的作用。我们实验室前期研究发现BC027988在肝癌组织中表达上调，是一个肝癌细胞增殖、转移的促进因子。本课题将重点研究BC027988在肝癌组织中的表达情况及其临床意义；BC027988在肝癌发生发展中的功能及其在肝癌中的表达调控机制；为深入理解肝癌发病的分子机制提供实验依据，为肝癌的治疗提供新的干预策略。