月季液泡氢离子P-ATPase转运蛋白及其作用成色的分子机制

Molecular breeding is an important way to produce Rose cutting flower cultivars. P-ATPase gene family play a role in morphological genesis, metabolism ,stress response to environments and so on. In the present study, we will isolate different P-ATPase genes in Rosa hybrida via transcriptome sequencing, bioinformatics analysis and other molecular cloning technology. On the other hand, based on various flower color mutants generated from our previous studies, transcriptsome information and analysis of gene expression, sub-cellular localization, yeast complementation and gene transformation, color related P-ATPas genes will be identified and isolated. Through structure comparison in different species, H+ P-ATPase transport protein identification, analyses of the relationships between them and nutrient utilization or pH value, and analyses to flower color related H+ P-ATPase functional mechanism, the diversity of P-ATPas gene structure and function, the evolution of color related P-ATPase genes and the gene network of key P-ATPase genes will be elucidated. This study will not only elucidate the putative function of varied types of P-ATPases genes in Rose and their diversity in pH value and flower color control, but also generate lots of gene resources for the genetic improvement in the woody plant. At the same time, the plant materials with high economic and research value will also be created, which are mainly related with nutrient efficient utilization, high tolerance to environmental stress or novel blue flower color in Rose.

分子育种是创建优质月季品种的重要手段之一。P-ATPase家族基因具有调控发育、次生代谢和抗性等重要功能。本项目一方面利用转录组测序、生物信息学及分子克隆等技术克隆月季不同结构的该家族基因；另一方面以前期研究所得的花色突变体为基础，利用基因表达、亚细胞定位及转基因分析确认与细胞pH值、花色等相关的P-ATPase家族基因。通过该家族基因的跨物种结构分析，月季液泡氢离子P-ATPase转运蛋白的识别及其与营养高效、液泡pH值的关联分析，色泽相关P-ATPases调控机制的分析，阐述月季该家族基因的结构和功能分化，明确与液泡pH、花色相关的P-ATPases分子进化及其作用机制。项目实施不仅可以诠释月季P-ATPase家族基因涉及的功能范围，而且可以明确其在调控液泡pH值与花色上的分化幅度。同时从实践上储备基因资源，有望创建一批营养高效、抗性强及蓝色花等具有较高产业和科研价值的材料。

分子育种是创建优质月季品种的重要手段之一。P-ATPase家族基因具有调控发育、次生代谢和抗性等重要功能。本项目一方面利用转录组测序、生物信息学及分子克隆等技术克隆月季不同结构的该家族基因；另一方面以前期研究所得的花色突变体为基础，利用基因表达、亚细胞定位及转基因分析确认与细胞pH值、花色等相关的P-ATPase家族基因。通过项目的实施，获得以月季资源代表种的各器官表达基因信息平台,综合分析了月季P-ATPases家族基因及与花色相关基因的表达相关性。完成了月季部分基因的亚细胞定位及转基因分析。明确了部分月季中与花色密切相关的P-ATPases家族基因，并得到它们的上游调控候选基因。项目中的全基因表达谱，可广泛用于基因表达的研究。通过成色相关转运蛋白研究打破成色物质转运的原有认识，为人为调控月季的花色提供理论基础。项目同时储备了基因资源，建立了一批对该研究极具有科研价值和高产业价值的植物转基因材料。