月季及其近缘种苯丙氨酸途径色香代谢关联的分子调控机制

Molecular breeding is becoming an important way to produce Rose and other woodplant cultivars. Phenylalanine metabolism is a key pathway to generate the compounds related to color and scents. In the present study, based on the collected Rosa hybrida and relative species, spontaneous and transgenic muatant, the genes related to phenylalanine metabolism will be isolated and the branches will be defined using comparative genomics, transcriptome, metabonomics and molecular biology methods. On the other hand, through the transgenic analysis via over-expression and knockout the key genes of phenylalanine metabolism pathway in plants, metablites quantitative and transcriptome analysis, the downstream responsive genes to those key genes are identified and the function of them will be studied in order to illustrate the molecular regulation mechanism of those key genes. Through investigation of the relationship between the key genes responding to the formation of anthocyanidin and scent compounds in phenylalanine metabolism, the molecular regulation mechanism to control these metabolic flux will be elucidated. After the implementation of this project, the association molecular regulation mechanism to control anthocyanidin and scent compounds formation will be known, and gene resources for the genetic improvement are achieved. At the same time, the plant materials with high economic and research value will also be created , which are with vivid color, favorite scent and easily cultivated in Rose.

分子育种是月季等木本植物的重要育种手段。苯丙氨酸代谢途径是植物色香物质合成的主要通路。本项目一方面以前期收集的月季及其近缘种资源、以及创建的转基因材料为基础，通过比较基因组学、转录组学、代谢组学等系统生物学和分子生物技术克隆月季及其近缘种苯丙氨酸代谢途径色香代谢的相关调控及结构基因，并确认各代谢支路的关键基因。另一方面，通过对色香代谢关键基因的超量表达及敲除分析，转基因材料代谢物定量分析及其转录组分析识别其下游响应基因，并对主要受控基因进行功能诊断，阐述色香代谢关键基因的分子作用机制。进一步结合对转基因材料代谢物累积及外源处理的反馈控制的研究，明确月季及其近缘种中苯丙氨酸途径色香代谢关联的分子调控机制。项目的实施不仅可以增进对植物色香关联调控机制的认识，而且从实践上储备了基因资源，有望创建一批色香兼备且易于栽培等的具有较高科研和经济价值的材料。

分子育种是月季等木本植物的重要育种手段。苯丙氨酸代谢途径是植物色香物质合成的主 要通路。本项目主要内容为克隆月季及其近缘种苯丙氨酸代谢途径色香代谢的相关调控及结构基因与阐述色香代谢关键基因的分子作用机制。通过项目实施，我们得到了月季和玫瑰代表种全器官、及其部分品种、近缘种特定花发育时期的表达基因资源平台，获取了月季及其近缘种苯丙氨酸代谢途径各代谢流可能存在的调控与结构基因，并明确了其关键基因的分子作用机理。项目实施后，不仅可以增进对植物色香关联调控机制的认识，而且从实践上储备了基因资源，有望创建一批色香兼备且易于栽培等的具有较高科研和经济价值的材料, 进一步为月季及其它木本花卉育种提供基础。