转NTF基因的SC修复脊髓损伤的实验研究

Fetal spinal cord cells, Schwann cells and neurotrophins all have the capacity to promote repair of injured spinal cord in animal models. To explore the possibility of using these approaches to treat clinical patients, we have examined whether a combination of these protocols is more effective than one protocol alone. The spinal cord of adult rats (136) was injured with the modified Allen's method. One week after injury, the injured cords were injected with Dubecco-modified Eagles Medium (DMEM, Group I), fetal spinal cord cell suspension (FSCS, Group II), FSCS plus Schwann cells (Group III), FSCS plus Schwann cells over-expressing nerve growth factor (NGF) (Group IV), FSCS plus Schwann cells over-expressing brain-derived neurotrophic factor (BDNF) (Group V), or FSCS plus Schwann cells over-expressing both NGF and BDNF (Group VI). One and three months after grafting, rats were subjected to behavioral tests and injured spinal cords were processed for light and electronic microscopy. Anterograde tracing of corticospinal tract was performed in some of these rats (n=16). Effects of co-grafts of FSCS with genetically modified Schwann cells over-expressing NGF and BDNF (Group VI) were superior to those of any other groups on the promotion of spinal cord repair. The co-grafts significantly enhanced functional recovery and elicited a robust growth of corticospinal axons and axonal myelination. Grafted Schwann cells and fetal spinal cord cells were well integrated within the host and promoted neurofilament, 5-HT and myelin basic protein immunoreactivity in injured cords. It is concluded that the combination of FSCS with genetically modified Schwann cells over-expressing NGF and BDNF was the most effective protocol to treat spinal cord injury, and this protocol may be useful for treatment of clinical patients with acute spinal cord injury. .Keywords: Schwann cell, fetal spinal cord suspension, CNS regeneration, nerve growth factor, brain-derived neurotrophic factor.Fetal spinal cord cells, Schwann cells and neurotrophins all have the capacity to promote repair of injured spinal cord in animal models. To explore the possibility of using these approaches to treat clinical patients, we have examined whether a combination of these protocols is more effective than one protocol alone. The spinal cord of adult rats (136) was injured with the modified Allen's method. One week after injury, the injured cords were injected with Dubecco-modified Eagles Medium (DMEM, Group I), fetal spinal cord cell suspension (FSCS, Group II), FSCS plus Schwann cells (Group III), FSCS plus Schwann cells over-expressing nerve growth factor (NGF) (Group IV), FSCS plus Schwann cells over-expressing brain-derived neurotrophic factor (BDNF) (Group V), or FSCS plus Schwann cells over-expressing both NGF and BDNF (Group VI). One and three months after grafting, rats were subjected to behavioral tests and injured spinal cords were processed for light and electronic microscopy. Anterograde tracing of corticospinal tract was performed in some of these rats (n=16). Effects of co-grafts of FSCS with genetically modified Schwann cells over-expressing NGF and BDNF (Group VI) were superior to those of any other groups on the promotion of spinal cord repair. The co-grafts significantly enhanced functional recovery and elicited a robust growth of corticospinal axons and axonal myelination. Grafted Schwann cells and fetal spinal cord cells were well integrated within the host and promoted neurofilament, 5-HT and myelin basic protein immunoreactivity in injured cords. It is concluded that the combination of FSCS with genetically modified Schwann cells over-expressing NGF and BDNF was the most effective protocol to treat spinal cord injury, and this protocol may be useful for treatment of clinical patients with acute spinal cord injury. .Keywords: Schwann cell, fetal spinal cord suspension, CNS regeneration, nerve growth factor, brain-derived neurotrophic factor.Fetal spinal cord cells, Schwann cells and neurotrophins all have the capacity to promote repair of injured spinal

拟在胚胎脊髓悬液移植基础上，将NTF家族不同功能的基因导入雪旺氏细胞植入脊髓损伤动锬Ｐ停嗷ゴ俳餐薷矗鄄霤GRP，GFAP，NF和脊髓组织形态学改变，原位杂交定位桶攵糠治鯪TFMRNA阳性神经元数量，SEP和tarlov运动分级，评价脊髓功能恢复程度，从蚪嵌忍教种瘟萍顾杷鹕擞行浴?.