辣椒抗黄瓜花叶病毒主效基因的图位克隆

Cucumber mosaic virus (CMV) is one of the most overrepresented viruses in pepper that makes great harm to the yield, quality and economic benefit of pepper. CMV, spreading rapidly and widely, has become more and more serious in recent years. Until now, pepper lacks resources with high-resistance to CMV, and the CMV resistance of pepper varieties in the domestic market needs to be improved. Therefore, CMV-resistance breeding is an urgent need. Concerning this problem, we have screened many pepper germplasm resources, and obtained an accession Pep-72, showing high resistance to CMV, but its CMV-resistance gene(s) are still unknown. We will conduct the cloning of the major gene conferring CMV resistance using the QTL-seq, traditional genetic linkage analysis combined with bioinformatics, expression analysis, and VIGS. The completion of this project can reveal the genetic basis of CMV-resistance in Pep-72, and lay a foundation for analyses on the molecular mechanism of CMV-resistance in pepper. In addition, this work will accelerate the breeding of the new CMV-resistant varieties of pepper in the future.

黄瓜花叶病毒（Cucumber mosaic virus，CMV）是危害辣椒生产的主要病害，严重威胁辣椒的产量、品质和经济效益。由于CMV影响范围广，传播速度快，近年来其危害日趋严重。而至今，辣椒CMV高抗资源十分缺乏，尤其是国内栽培品种CMV抗性仍需提高，辣椒CMV抗病育种形势严峻。针对该问题，本项目组前期从大量辣椒资源中筛选到对CMV-cas表现高抗材料Pep-72，其抗性基础亟待研究。本研究拟利用QTL-seq、传统遗传连锁分析结合生物信息学、表达分析、VIGS等手段，完成CMV抗病主效基因的克隆。该项目的完成可阐明辣椒Pep-72的CMV抗性的遗传基础，为解析辣椒CMV抗性的分子机制奠定基础，同时也对今后辣椒CMV抗病资源挖掘，培育辣椒CMV抗病新品种提供借鉴和参考。

黄瓜花叶病毒（Cucumber mosaic virus，CMV）是危害辣椒生产的主要病害，严重威胁辣椒的产量、品质和经济效益。由于CMV影响范围广，传播速度快，近年来其危害日趋严重。而至今，辣椒CMV高抗资源十分缺乏，尤其是国内栽培品种CMV抗性仍需提高，辣椒CMV抗病育种形势严峻。本项目利用QTL-seq、传统遗传连锁分析最终将CMV-cas抗性主效QTL Pep CMV3.1定位在标记P1733-P1764之间，物理位置17,328,953bp~ 17,644,774bp区间内，物理距离约为310Kb；基于基因功能注释和变异分析确定了2个基因CA03g06080和CA03g06090为CMV抗性候选基因；明确了我国辣椒主产区主要致病CMV株系类型属于CMV-IB亚组；在300余份辣椒核心种质中筛选出高抗（HR）和免疫（I）CMV的种质资源材料17份。上述内容为今后辣椒CMV抗病基因克隆、抗病机理解析和辣椒CMV抗病品种培育奠定理论和材料基础。