BOPHY及其类似物的合成与性质研究

The develpoment of organic fluoresecent dyes is the foundation for the innovation in biomedical and materials science.BOPHY dyes as a newly developed (by us and Ziegler's group) organic fluorescent dyes have received wide interests due to their efficient synthesis and functionalization methods and their excellent photophysical properties, and have found applications as energy transfer cascade, photovoltaics, fluorescent probes and photosensitizers. Currently, their further application in biomedical science, especially in fluorescent imaging areas have been hampered by their poor water solubility and relatively short absorption wavelength. In this project, we will develop efficient synthetic methods for the efficient acccess to water-soluble and/or long-wavelength absorbing BOPHY dyes, synthesize novel D-A-D type BOPHY derivatives and their unsymmetrical analogs as long wavelength and/or two photon absorbing dyes, and develop several novel BOPHY probes with good water-solubility and biocompatibility. This project will promote the further application of BOPHY dyes in biomedical science such as fluorescence imaging area.

有机荧光染料的发展是生物医学和材料科学领域创新的基础。BOPHY染料作为一类刚刚发展起来新型荧光团,具有简洁高效的合成衍生方法和优异的光学性质，迅速得到广泛关注。在过去一年多来，已被应用于构建能量转移试剂盒、太阳能电池、荧光探针和光敏剂等方面。目前限制该类分子在生物医学方面，尤其是荧光成像等领域应用的主要因素是缺乏具有良好水溶性和/或长波吸收发射的衍生物分子。本课题旨在发展高效合成及修饰衍生BOPHY分子的新方法,构建具有良好水溶性的BOPHY衍生物;通过推拉电子效应，设计合成具有长波吸收发射及双光子吸收性质的系列新型D-A-D型BOPHY衍生物和具有不对称结构的新型BOPHY类似物;发展具有良好溶解性、靶向性、生物相容性的BOPHY（双光子）荧光探针。本项目的开展将推进BOPHY类新兴染料在荧光成像等生物医学相关领域的应用。

有机荧光染料的发展是生物医学和材料科学领域创新的基础。发展在易修饰衍生、生物相容性好、光谱可调的小分子荧光染料具有重要的科学意义和良好的应用前景。本项目发展了几种衍生BOPHY和BODIPY荧光染料分子的高效合成方法，构建系列BOPHY衍生物；设计合成了几种具有新型不对称BOPHY类似物(BOPPY, BOAHY, BOAPY, BOHPY, BOHZPY, BOBPY等)；研究系列新染料的光谱性质、电化学性质以及光物理化学稳定性，并由此构建了几种具有良好溶解性、靶向性、生物相容性好的小分子染料探针。迄今为止，本项目培养毕业研究生12名，毕业本科生18名；项目成果获授权国家发明专利5项，发表SCI收录研究论文全文41篇。本项目的成果将为新型功能荧光染料和探针的设计合成和应用提供有效推动。