肠道菌群和粘膜适应性平衡与溃疡性结肠炎的研究

Our research focuses on the core relation among intestinal bacteria, mucosal immune imbalance and UC .Aim to find the relevant bacteria or core flora of UC. Here, we compare mucous membrane of bacteria group and mucosal immune development of the five rat groups which are feed with different diets immediately after they are weaned。Many investation had found the evidence of alteration of intestinal flora. In our study, the main methods we used to find the relevant bacteria is to detect V3-5 sequence of bacterial 16rRNA genes .According to the consistency of sequence analysis of OTU composition and frequency, relevant bacteria may be confirmed. Also evidence is emerging that the underlying mechanisms of intestinal epithelial tolerance versus intolerance towards luminal bacterial ligands are distinctly mediated through Toll-like receptors (TLRs) and other pattern recognition receptors of the innate immune system. The proportion of Treg cell and effective cell in lamina propria can be detected to reflect the different development of intestinal immune system. Also, UC model was established to further verify the above results. According to the results, we aim to find one or two relevant bacteria and clarify the relation between the relevant bacteria with mucosal immunity cells, which will benefit the further study of UC.

以肠道细菌与黏膜适应性平衡破坏可能与UC的发生有关为核心，以找到与UC相关的细菌种或核心菌群为目标。采用断奶后立即给乳鼠喂饲不同的饮食，检测黏膜细菌群和黏膜免疫发育与UC易感性的关系。细菌群的分析采用检测细菌16rRNA基因V3-5变区序列，分析序列一致性的OUT组成和频率，找到相关细菌，可为培养细菌进行选择性分离。黏膜免疫采用检测黏膜内细胞因子谱所构成的细胞因子微环境，固有层中调节T细胞（Treg）和效应T细胞群的比例，分析不同饮食喂饲成长的小鼠，肠内菌群组成和黏膜免疫特点与UC易感性关系。根据这一特点，建立UC模型进一步验证研究结果，并在不同期的UC患者中有目标地检测相关菌及黏膜免疫学指标。期望找到一至两种UC的相关菌并阐明其与黏膜免疫调节细胞间的关系，用于UC的防治和发生机理的深入研究。