基于3D打印温敏微载体规模化扩增间充质干细胞机制及方法研究

The researches on Mesenchymal Stem Cells (MSCs)-related products and drugs have a great potential to solve the medical issues caused by aging. The fate of stem cells is determined by their local microenvironment, therefore, the microcarriers play a critical role in guaranteeing the effective expansion of MSCs. However, few microcarriers have been established to be effective for large-scale expansion of MSCs. In the present study, we proposed a new approach to construct biomimetic and thermosensitive microcarriers by using photo-crosslinking 3D printing technology for the large-scale expansion of MSCs. By our approach, the expanded MSCs maintain the stemness, as well as intactly detach from the microcarriers without using trypsin. We believe that this research will provide a new way for the development of stem cell technology, and give the technical support for MSCs application on aging related-degenerative therapies in future.

研究开发以间充质干细胞（Mesenchymal Stem Cells，MSCs）为基础的产品和药物，对解决未来老龄化带来的医学问题具有重要意义。由于微环境决定干细胞命运，所以微载体是决定MSCs有效扩增的关键因素之一，但目前缺乏MSCs扩增特定的微载体。本项目申请拟通过模拟MSCs体内微环境，基于光固化3D打印技术制作一种新型仿生温敏微载体用以实现MSCs的规模化有效扩增、功能维持及温和有效脱附。本项目的实施将为干细胞技术的探索和发展提供一种新的思路，也为MSCs满足临床难治性退行性疾病的治疗提供技术保障。

研究开发以间充质干细胞（Mesenchymal Stem Cells，MSCs）为基础的产品和药物，对解决未来老龄化带来的医学问题具有重要意义。由于微环境决定干细胞命运，所以微载体是决定MSCs有效扩增的关键因素之一，但目前缺乏MSCs扩增特定的微载体。本项目通过模拟MSCs体内微环境，探寻MSCs高密度粘附与增殖的新途径。在明确促进MSCs粘附和增殖的有效成分及其作用机制前提下，探索适用于MSCs体外培养的水凝胶微载体及其方法，构建了一种可利用光固化3D打印技术制备的三维培养的水凝胶微载体，该水凝胶微载体可为细胞提供与体内更接近的培养环境，更加有利于细胞粘附、增殖、收获和冻存。在此基础上，课题组利用MSCs在水凝胶微载体上贴壁、增殖、功能维持与温和脱附的培养特性，建立起一套MSCs三维培养体系及其标准工艺流程，成功实现了MSCs规模化有效培养，配套的细胞培养操作方法简单快速高效，易于未来普及及规模化生产。本项目的顺利完成将为干细胞技术的探索和发展提供工具，也为MSCs满足临床难治性退行性疾病的治疗提供了坚实的技术保障。