基于lncRNA-miRNA相互调控网络探讨竹黄颗粒治疗银屑病的作用机制

Psoriasis (Psoriasis, PS) is a common chronic skin disease which is clinically intractable and recurrent.It has been found that susceptibility genes, microRNA, cytokines as well as their interaction network contribute to the outbreak of Psoriasis. However, the mechanism of upstream regulation of miRNA is still unclear. Our recent researches indicate that lncRNA is a key regulation factor of miRNA and interactions exist between them. Therefore, we propose a hypothesis that the regulatory network between lncRNA and miRNA regulates the expressions of downstream target genes and cytokines, which leads to the outbreak of Psoriasis. In this proposal, we will utilize advanced biochip technology to screen out lncRNA with specific expressions and construct the regulatory network between lncRNA and miRNA combining the previous MiRNAs with specific expressions. Also, the Zhuhuang granule will be used to verify the established regulatory network above. The search of this proposal will provide the pathogenesis of Psoriasis and evolution with new evidence, as well as to offer empirical evidences and pharmaceutical target spots for the treatment of psoriasis by traditional Chinese medicine.

银屑病是临床常见的慢性复发性难治性皮肤病，课题组前期研究发现，特异性miRNA、易感基因和细胞因子及其相互作用网络可能参与银屑病的发病，然而miRNA上游调控机制尚未明确。新近研究表明，lncRNA是miRNA的重要调控因子，且存在相互作用。因此，我们提出“lncRNA和miRNA相互作用，形成非编码RNA的复杂调控网络，共同调控下游的靶基因及细胞因子，最终导致银屑病的发生，且中药复方通过多靶点、多环节的网络调控而治疗银屑病”的科学假说。本项目拟采用前沿的生物芯片技术筛选特异性表达的lncRNA，结合前期特异性表达的miRNA构建lncRNA与miRNA相互调控网络，并采用确有疗效的竹黄颗粒确证lncRNA与miRNA相互调控网络，为银屑病发病及演变提供新的证据，为中医药治疗银屑病提供新的实证依据及药物靶点。

银屑病是临床常见的慢性复发性难治性皮肤病，课题组前期研究发现，特异性miRNA、易感基因和细胞因子及其相互作用网络可能参与银屑病的发病，然而miRNA上游调控机制尚未明确。新近研究表明，lncRNA是miRNA的重要调控因子，且存在相互作用。因此，我们提出lncRNA和miRNA相互作用，形成非编码RNA的复杂调控网络，共同调控下游的靶基因及细胞因子，最终导致银屑病的发生，且中药复方通过多靶点、多环节的网络调控而治疗银屑病。本项目通过前沿的生物芯片技术筛选特异性表达的lncRNA，结合前期特异性表达的miRNA构建CTNNBIP1、lncRNA SPRR2C/miR-330/STAT1和S100A7、lncRNA SH3PXD2A-AS1/miR-125b/STAT3、miR-20a/STAT3相互调控网络，并采用确有疗效的竹黄颗粒确证lncRNA与miRNA相互调控网络，为银屑病发病及演变提供新的证据，为中医药治疗银屑病提供新的实证依据及药物靶点。